Matches in SemOpenAlex for { <https://semopenalex.org/work/W2735014630> ?p ?o ?g. }
- W2735014630 endingPage "4126" @default.
- W2735014630 startingPage "4117" @default.
- W2735014630 abstract "Ribosomes are present inside bacterial cells at micromolar concentrations and occupy up to 20% of the cell volume. Under these conditions, even weak quinary interactions between ribosomes and cytosolic proteins can affect protein activity. By using in-cell and in vitro NMR spectroscopy, and biophysical techniques, we show that the enzymes, adenylate kinase and dihydrofolate reductase, and the respective coenzymes, ATP and NADPH, bind to ribosomes with micromolar affinity, and that this interaction suppresses the enzymatic activities of both enzymes. Conversely, thymidylate synthase, which works together with dihydrofolate reductase in the thymidylate synthetic pathway, is activated by ribosomes. We also show that ribosomes impede diffusion of green fluorescent protein in vitro and contribute to the decrease in diffusion in vivo. These results strongly suggest that ribosome-mediated quinary interactions contribute to the differences between in vitro and in vivo protein activities and that ribosomes play a previously under-appreciated nontranslational role in regulating cellular biochemistry." @default.
- W2735014630 created "2017-07-21" @default.
- W2735014630 creator A5055881066 @default.
- W2735014630 creator A5084202200 @default.
- W2735014630 creator A5085908199 @default.
- W2735014630 creator A5089224444 @default.
- W2735014630 creator A5089822974 @default.
- W2735014630 date "2017-08-03" @default.
- W2735014630 modified "2023-10-12" @default.
- W2735014630 title "Ribosome Mediated Quinary Interactions Modulate In-Cell Protein Activities" @default.
- W2735014630 cites W1502372250 @default.
- W2735014630 cites W1543817477 @default.
- W2735014630 cites W1570895488 @default.
- W2735014630 cites W1591386696 @default.
- W2735014630 cites W1788190869 @default.
- W2735014630 cites W1883779479 @default.
- W2735014630 cites W1898197987 @default.
- W2735014630 cites W1941479381 @default.
- W2735014630 cites W1948637354 @default.
- W2735014630 cites W1970726343 @default.
- W2735014630 cites W1975497257 @default.
- W2735014630 cites W1981981820 @default.
- W2735014630 cites W1985161570 @default.
- W2735014630 cites W1986606329 @default.
- W2735014630 cites W1987730349 @default.
- W2735014630 cites W1989161865 @default.
- W2735014630 cites W1994460797 @default.
- W2735014630 cites W1994751660 @default.
- W2735014630 cites W2001507431 @default.
- W2735014630 cites W2005282689 @default.
- W2735014630 cites W2007375228 @default.
- W2735014630 cites W2011456814 @default.
- W2735014630 cites W2013439046 @default.
- W2735014630 cites W2015580675 @default.
- W2735014630 cites W2017934591 @default.
- W2735014630 cites W2022394654 @default.
- W2735014630 cites W2022437289 @default.
- W2735014630 cites W2027719220 @default.
- W2735014630 cites W2041440237 @default.
- W2735014630 cites W2045389620 @default.
- W2735014630 cites W2046535391 @default.
- W2735014630 cites W2053328000 @default.
- W2735014630 cites W2056164591 @default.
- W2735014630 cites W2062635576 @default.
- W2735014630 cites W2068245217 @default.
- W2735014630 cites W2068457504 @default.
- W2735014630 cites W2076191780 @default.
- W2735014630 cites W2076391704 @default.
- W2735014630 cites W2078016208 @default.
- W2735014630 cites W2078306077 @default.
- W2735014630 cites W2084273798 @default.
- W2735014630 cites W2085226685 @default.
- W2735014630 cites W2088412411 @default.
- W2735014630 cites W2094604396 @default.
- W2735014630 cites W2118445471 @default.
- W2735014630 cites W2119117290 @default.
- W2735014630 cites W2123254545 @default.
- W2735014630 cites W2126309335 @default.
- W2735014630 cites W2127068865 @default.
- W2735014630 cites W2132392209 @default.
- W2735014630 cites W2135386829 @default.
- W2735014630 cites W2142417893 @default.
- W2735014630 cites W2157143879 @default.
- W2735014630 cites W2159394948 @default.
- W2735014630 cites W2160594875 @default.
- W2735014630 cites W2161740289 @default.
- W2735014630 cites W2169242133 @default.
- W2735014630 cites W2169787271 @default.
- W2735014630 cites W2226425375 @default.
- W2735014630 cites W2238207809 @default.
- W2735014630 cites W2253387945 @default.
- W2735014630 cites W2293953879 @default.
- W2735014630 cites W2312471577 @default.
- W2735014630 cites W2436261417 @default.
- W2735014630 cites W2506406652 @default.
- W2735014630 cites W2565163836 @default.
- W2735014630 cites W2621126205 @default.
- W2735014630 cites W2949250147 @default.
- W2735014630 cites W4234175308 @default.
- W2735014630 cites W4235129795 @default.
- W2735014630 cites W4238701569 @default.
- W2735014630 cites W4239833133 @default.
- W2735014630 cites W4292408570 @default.
- W2735014630 cites W4298057496 @default.
- W2735014630 doi "https://doi.org/10.1021/acs.biochem.7b00613" @default.
- W2735014630 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5956540" @default.
- W2735014630 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28715177" @default.
- W2735014630 hasPublicationYear "2017" @default.
- W2735014630 type Work @default.
- W2735014630 sameAs 2735014630 @default.
- W2735014630 citedByCount "30" @default.
- W2735014630 countsByYear W27350146302017 @default.
- W2735014630 countsByYear W27350146302018 @default.
- W2735014630 countsByYear W27350146302019 @default.
- W2735014630 countsByYear W27350146302020 @default.
- W2735014630 countsByYear W27350146302021 @default.
- W2735014630 countsByYear W27350146302022 @default.
- W2735014630 countsByYear W27350146302023 @default.