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- W2735330110 abstract "A reverse phase liquid chromatographic method for the simultaneous determination of lamivudine and abacavir in pure and tablet formulation was developed and validated. The method was found to simple, precise and accurate. The separation was carried out using Phenomenex C18 (250 x 4.6 mm, 5 μm particle size) column, with a mobile phase consisting of phosphate buffer (pH 7.8) and methanol in the ratio of 50:50 % v/v. The flow rate was set at 1.0 mL/min and detection was monitored at 216 nm. The retention times of lamivudine and abacavir were found to be 3.147 and 6.367 min, respectively. The linearity was found in the concentration range of and 80-280 μg/mL and 75-450 μg/mL for lamivudine and abacavir, respectively. The liquid chromatography method was extensively validated for linearity, accuracy, precision, and robustness. All these analytical validation parameters were found satisfactory and the % RSD was determined which indicates the usefulness of method for determination of lamivudine and abacavir in bulk drug and tablet formulation." @default.
- W2735330110 created "2017-07-21" @default.
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- W2735330110 date "2013-01-01" @default.
- W2735330110 modified "2023-09-24" @default.
- W2735330110 title "Method Development and Validation of aReverse phase Liquid ChromatographicMethod for Simultaneous Determination ofLamivudine and Abacavir Sulphate inTablets" @default.
- W2735330110 hasPublicationYear "2013" @default.
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