Matches in SemOpenAlex for { <https://semopenalex.org/work/W2735407103> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2735407103 endingPage "678" @default.
- W2735407103 startingPage "678" @default.
- W2735407103 abstract "3 The endothelium regulates vascular tone through the release of relaxing factors including nitric oxide, prostacyclin, epoxyeicosatrienoic acids and others not yet identified. The purpose of this study was to characterize the 15-lipoxygenase product, 11,12,15-trihydroxyeicosatrienoic acid (11,12,15-THETA), as a new endothelial relaxing factor. In phenylephrine-constricted rabbit aorta, acetylcholine induced a dose-dependent relaxation in the presence of cyclooxygenase blockade with indomethicin (10 μM) and nitric oxide blockade with nitro-arginine (30 μM) which was eliminated by pretreatment with cinnamyl-3,4,-dihydroxy-a-cyanocinnamate (CDC)(1 μM) a specific lipoxygenase inhibitor, and miconizole (1 μM) a cytochrome P450 inhibitor. Additionally, arachidonic acid (1 nM - 10 μM) relaxed precontracted rabbit aorta, and this relaxation was maximally inhibited by 50% either by increasing the potassium (K + ) concentration from 4 mM to 20 mM or by the addition of the K + channel blockers, apamin (100 nM) plus charybdotoxin (100 nM). In patch-clamp studies of inside-out patches of rabbit aortic smooth muscle, we have identified a 20 pS K + channel, which is activated by increased calcium concentrations and blocked by apamin (1 μM). Structural analysis of arachidonic acid metabolites from rabbit aorta by gas chromatography/mass spectometry has revealed two 15-lipoxygenase product regioisomers; 11,12,15-THETA and 11,14,15-THETA. The synthesis of these metabolites was inhibited by CDC and miconizole. The addition of the 11,12,15-THETA fraction to precontracted rabbit aorta resulted in a concentration-dependent relaxation, which was blocked by elevated K + . The other regioisomer was without effect. These results suggest that 11,12,15-THETA, a 15-lipoxygenase metabolite, represents a novel endothelium-derived relaxing factor, which induces relaxation possibly through the activation of apamin-sensitive K + channels located in the vascular smooth muscle." @default.
- W2735407103 created "2017-07-21" @default.
- W2735407103 creator A5000214187 @default.
- W2735407103 creator A5014603417 @default.
- W2735407103 creator A5064720938 @default.
- W2735407103 creator A5067916190 @default.
- W2735407103 creator A5090046945 @default.
- W2735407103 date "2000-10-01" @default.
- W2735407103 modified "2023-09-26" @default.
- W2735407103 title "Role of 11,12,15-Trihydroxyeicosatrienoic Acid as a New Endothelium-Derived Relaxing Factor" @default.
- W2735407103 doi "https://doi.org/10.1161/01.hyp.36.suppl_1.678-b" @default.
- W2735407103 hasPublicationYear "2000" @default.
- W2735407103 type Work @default.
- W2735407103 sameAs 2735407103 @default.
- W2735407103 citedByCount "0" @default.
- W2735407103 crossrefType "journal-article" @default.
- W2735407103 hasAuthorship W2735407103A5000214187 @default.
- W2735407103 hasAuthorship W2735407103A5014603417 @default.
- W2735407103 hasAuthorship W2735407103A5064720938 @default.
- W2735407103 hasAuthorship W2735407103A5067916190 @default.
- W2735407103 hasAuthorship W2735407103A5090046945 @default.
- W2735407103 hasConcept C126322002 @default.
- W2735407103 hasConcept C134018914 @default.
- W2735407103 hasConcept C178790620 @default.
- W2735407103 hasConcept C181199279 @default.
- W2735407103 hasConcept C185592680 @default.
- W2735407103 hasConcept C2776919887 @default.
- W2735407103 hasConcept C2776992346 @default.
- W2735407103 hasConcept C2777952589 @default.
- W2735407103 hasConcept C2778078955 @default.
- W2735407103 hasConcept C2779395532 @default.
- W2735407103 hasConcept C2779411790 @default.
- W2735407103 hasConcept C2779689624 @default.
- W2735407103 hasConcept C2779980429 @default.
- W2735407103 hasConcept C2780419564 @default.
- W2735407103 hasConcept C2780857362 @default.
- W2735407103 hasConcept C2992686903 @default.
- W2735407103 hasConcept C519581460 @default.
- W2735407103 hasConcept C55493867 @default.
- W2735407103 hasConcept C71924100 @default.
- W2735407103 hasConcept C83743174 @default.
- W2735407103 hasConcept C84393581 @default.
- W2735407103 hasConcept C86803240 @default.
- W2735407103 hasConceptScore W2735407103C126322002 @default.
- W2735407103 hasConceptScore W2735407103C134018914 @default.
- W2735407103 hasConceptScore W2735407103C178790620 @default.
- W2735407103 hasConceptScore W2735407103C181199279 @default.
- W2735407103 hasConceptScore W2735407103C185592680 @default.
- W2735407103 hasConceptScore W2735407103C2776919887 @default.
- W2735407103 hasConceptScore W2735407103C2776992346 @default.
- W2735407103 hasConceptScore W2735407103C2777952589 @default.
- W2735407103 hasConceptScore W2735407103C2778078955 @default.
- W2735407103 hasConceptScore W2735407103C2779395532 @default.
- W2735407103 hasConceptScore W2735407103C2779411790 @default.
- W2735407103 hasConceptScore W2735407103C2779689624 @default.
- W2735407103 hasConceptScore W2735407103C2779980429 @default.
- W2735407103 hasConceptScore W2735407103C2780419564 @default.
- W2735407103 hasConceptScore W2735407103C2780857362 @default.
- W2735407103 hasConceptScore W2735407103C2992686903 @default.
- W2735407103 hasConceptScore W2735407103C519581460 @default.
- W2735407103 hasConceptScore W2735407103C55493867 @default.
- W2735407103 hasConceptScore W2735407103C71924100 @default.
- W2735407103 hasConceptScore W2735407103C83743174 @default.
- W2735407103 hasConceptScore W2735407103C84393581 @default.
- W2735407103 hasConceptScore W2735407103C86803240 @default.
- W2735407103 hasLocation W27354071031 @default.
- W2735407103 hasOpenAccess W2735407103 @default.
- W2735407103 hasPrimaryLocation W27354071031 @default.
- W2735407103 hasRelatedWork W1965202443 @default.
- W2735407103 hasRelatedWork W1982573306 @default.
- W2735407103 hasRelatedWork W1983556643 @default.
- W2735407103 hasRelatedWork W2015528248 @default.
- W2735407103 hasRelatedWork W2021580134 @default.
- W2735407103 hasRelatedWork W2066262518 @default.
- W2735407103 hasRelatedWork W2066957125 @default.
- W2735407103 hasRelatedWork W2066982642 @default.
- W2735407103 hasRelatedWork W2073292665 @default.
- W2735407103 hasRelatedWork W2074876742 @default.
- W2735407103 hasRelatedWork W2089594341 @default.
- W2735407103 hasRelatedWork W2092495512 @default.
- W2735407103 hasRelatedWork W2100581955 @default.
- W2735407103 hasRelatedWork W2110782817 @default.
- W2735407103 hasRelatedWork W2144486173 @default.
- W2735407103 hasRelatedWork W2324865880 @default.
- W2735407103 hasRelatedWork W2460980233 @default.
- W2735407103 hasRelatedWork W2744118485 @default.
- W2735407103 hasRelatedWork W2946364417 @default.
- W2735407103 hasRelatedWork W2993021879 @default.
- W2735407103 hasVolume "36" @default.
- W2735407103 isParatext "false" @default.
- W2735407103 isRetracted "false" @default.
- W2735407103 magId "2735407103" @default.
- W2735407103 workType "article" @default.