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- W2735448804 abstract "Diadenosine polyphosphates (ApnA, n = 2–6), particularly Ap4A, are involved in several important physiological processes. The substantial sequence identity of the Nudix hydrolase domain (domain 1) of Mycobacterium smegmatis MutT1 (MsMutT1) with a known Ap4A hydrolase suggested that MsMutT1 could also hydrolyse diadenosine polyphosphates. Biochemical experiments yielded results in conformity with this suggestion, with Ap4A as the best among the substrates. ATP is a product in all experiments; small amounts of ADP were also observed in the experiments involving Ap4A and Ap6A. Hydrolysis was inhibited by fluoride ions in all cases. The mechanism of action and its inhibition in relation to ApnA were explored through the X-ray analysis of the crystals of the MsMutT1 complexes with Ap5A; Ap5A and MnCl2; Ap4A; ATP; and ATP.NaF.MgCl2. The aggregation pattern of molecules in the first four crystals is similar to that found in a majority of MsMutT1-NTP crystals. Substrate molecules occupy the primary binding site and ATP occupies a site at an intermolecular interface, in the first two. ATP occupies both the sites in the third and fourth crystal. The protein-ligand interactions observed in these crystal structures lead to an explanation of the molecular mechanism of hydrolysis of ApnA by MsMutT1. The fifth crystal exhibits a new packing arrangement. The structure of the complex provides an explanation for the fluoride inhibition of the activity of the enzyme. It would thus appear that MutT1 has a major role involving the hydrolysis of diadenosine polyphosphates, which could be elucidated at the molecular level." @default.
- W2735448804 created "2017-07-21" @default.
- W2735448804 creator A5018607234 @default.
- W2735448804 creator A5026545921 @default.
- W2735448804 creator A5053819735 @default.
- W2735448804 date "2017-09-01" @default.
- W2735448804 modified "2023-10-17" @default.
- W2735448804 title "Hydrolysis of diadenosine polyphosphates. Exploration of an additional role of Mycobacterium smegmatis MutT1" @default.
- W2735448804 cites W113620132 @default.
- W2735448804 cites W141737879 @default.
- W2735448804 cites W1501272646 @default.
- W2735448804 cites W1680719178 @default.
- W2735448804 cites W1772562074 @default.
- W2735448804 cites W1782197742 @default.
- W2735448804 cites W1965022789 @default.
- W2735448804 cites W1965926170 @default.
- W2735448804 cites W1966761578 @default.
- W2735448804 cites W1967194316 @default.
- W2735448804 cites W1970879773 @default.
- W2735448804 cites W1976515062 @default.
- W2735448804 cites W1980407386 @default.
- W2735448804 cites W1985114988 @default.
- W2735448804 cites W1992761237 @default.
- W2735448804 cites W1995017064 @default.
- W2735448804 cites W1998709156 @default.
- W2735448804 cites W1999651926 @default.
- W2735448804 cites W2002366878 @default.
- W2735448804 cites W2007466227 @default.
- W2735448804 cites W2010189112 @default.
- W2735448804 cites W2022754746 @default.
- W2735448804 cites W2023910328 @default.
- W2735448804 cites W2025300024 @default.
- W2735448804 cites W2030224700 @default.
- W2735448804 cites W2033404481 @default.
- W2735448804 cites W2034373772 @default.
- W2735448804 cites W2043912723 @default.
- W2735448804 cites W2046052421 @default.
- W2735448804 cites W2047751135 @default.
- W2735448804 cites W2049125285 @default.
- W2735448804 cites W2053430990 @default.
- W2735448804 cites W2056015083 @default.
- W2735448804 cites W2056481747 @default.
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- W2735448804 cites W2078454267 @default.
- W2735448804 cites W2086947155 @default.
- W2735448804 cites W2086986542 @default.
- W2735448804 cites W2092632970 @default.
- W2735448804 cites W2102872728 @default.
- W2735448804 cites W2108921801 @default.
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- W2735448804 cites W2113201746 @default.
- W2735448804 cites W2120983223 @default.
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- W2735448804 cites W2128482141 @default.
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- W2735448804 cites W2159211495 @default.
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- W2735448804 cites W2165115532 @default.
- W2735448804 cites W2165914529 @default.
- W2735448804 cites W2169524332 @default.
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- W2735448804 cites W2602264965 @default.
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- W2735448804 doi "https://doi.org/10.1016/j.jsb.2017.07.002" @default.
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