FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2735475571> ?p ?o ?g. }

• W2735475571 endingPage "635" @default.
• W2735475571 startingPage "629" @default.
• W2735475571 abstract "An enhanced surface plasmon resonance (SPR) biosensor system using magnetic nanoparticles as amplifying element was developed for the real-time determination of estradiol (E2) based on indirect competition method. The anti-estradiol monoclonal antibody (E2-mAb) was conjugated with the magnetic nanoparticles (MNPs) via protein A for signal amplification to improve the detection sensitivity. The chitosan, spin-coated on the sensor chip surface, was used to immobilize the antigen (E2-BSA) by glutaraldehyde. The chitosan could significantly improve the performance and stability of the immobilized E2-BSA. During the detection, E2-BSA competed with E2 in samples for binding with the E2-mAb-MNPs conjugates. Then the SPR response decreased in the presence of E2 because E2 prevented the combination of E2-mAb-MNPs conjugates and E2-BSA. In other words, the response of SPR sensor was inversely proportional to E2 concentration. Assay parameters, such as the amount of antibody and MNPs, the amount of immobilized E2-BSA and E2-mAb-MNPs concentration, were optimized in detail and E2-spiked milk samples were detected. A good linear relationship was obtained between inhibition and lgC(E2) ranging from 1.95 to 2000 ng/mL and the limit of detection (LOD) was 0.81 ng/mL. The result implied that the detection sensitivity was improved compared with the traditionnal sensor without magnetic nanoparticles. Meanwhile, an ELISA method was conducted to detect E2 in milk as comparation with the enhanced SPR method, and the results indicated that these two methods had good consistency. The present study demonstrated a presumable general way and the enhanced sensors possessed a promising application for detection of various kinds of small molecules." @default.
• W2735475571 created "2017-07-21" @default.
• W2735475571 creator A5002633478 @default.
• W2735475571 creator A5012611475 @default.
• W2735475571 creator A5014459708 @default.
• W2735475571 creator A5028396253 @default.
• W2735475571 creator A5034337630 @default.
• W2735475571 creator A5041151211 @default.
• W2735475571 creator A5054195768 @default.
• W2735475571 creator A5063140774 @default.
• W2735475571 date "2018-01-01" @default.
• W2735475571 modified "2023-10-17" @default.
• W2735475571 title "Magnetic nanoparticle enhanced surface plasmon resonance sensor for estradiol analysis" @default.
• W2735475571 cites W1966267387 @default.
• W2735475571 cites W1970121975 @default.
• W2735475571 cites W1971375143 @default.
• W2735475571 cites W1976102031 @default.
• W2735475571 cites W1977689899 @default.
• W2735475571 cites W1996262658 @default.
• W2735475571 cites W2014424793 @default.
• W2735475571 cites W2019784326 @default.
• W2735475571 cites W2023372341 @default.
• W2735475571 cites W2023704290 @default.
• W2735475571 cites W2024218463 @default.
• W2735475571 cites W2025844719 @default.
• W2735475571 cites W2028186790 @default.
• W2735475571 cites W2032050990 @default.
• W2735475571 cites W2033463495 @default.
• W2735475571 cites W2035017448 @default.
• W2735475571 cites W2044684204 @default.
• W2735475571 cites W2045221143 @default.
• W2735475571 cites W2048655519 @default.
• W2735475571 cites W2051091991 @default.
• W2735475571 cites W2059595039 @default.
• W2735475571 cites W2075995140 @default.
• W2735475571 cites W2080926524 @default.
• W2735475571 cites W2082200596 @default.
• W2735475571 cites W2084526855 @default.
• W2735475571 cites W2086891790 @default.
• W2735475571 cites W2087725973 @default.
• W2735475571 cites W2109897848 @default.
• W2735475571 cites W2110435198 @default.
• W2735475571 cites W2110878030 @default.
• W2735475571 cites W2147005510 @default.
• W2735475571 cites W2159992904 @default.
• W2735475571 cites W2190623137 @default.
• W2735475571 cites W2191001621 @default.
• W2735475571 cites W2319960298 @default.
• W2735475571 doi "https://doi.org/10.1016/j.snb.2017.07.061" @default.
• W2735475571 hasPublicationYear "2018" @default.
• W2735475571 type Work @default.
• W2735475571 sameAs 2735475571 @default.
• W2735475571 citedByCount "62" @default.
• W2735475571 countsByYear W27354755712018 @default.
• W2735475571 countsByYear W27354755712019 @default.
• W2735475571 countsByYear W27354755712020 @default.
• W2735475571 countsByYear W27354755712021 @default.
• W2735475571 countsByYear W27354755712022 @default.
• W2735475571 countsByYear W27354755712023 @default.
• W2735475571 crossrefType "journal-article" @default.
• W2735475571 hasAuthorship W2735475571A5002633478 @default.
• W2735475571 hasAuthorship W2735475571A5012611475 @default.
• W2735475571 hasAuthorship W2735475571A5014459708 @default.
• W2735475571 hasAuthorship W2735475571A5028396253 @default.
• W2735475571 hasAuthorship W2735475571A5034337630 @default.
• W2735475571 hasAuthorship W2735475571A5041151211 @default.
• W2735475571 hasAuthorship W2735475571A5054195768 @default.
• W2735475571 hasAuthorship W2735475571A5063140774 @default.
• W2735475571 hasConcept C106847996 @default.
• W2735475571 hasConcept C119128265 @default.
• W2735475571 hasConcept C134306372 @default.
• W2735475571 hasConcept C155672457 @default.
• W2735475571 hasConcept C160756335 @default.
• W2735475571 hasConcept C171250308 @default.
• W2735475571 hasConcept C185592680 @default.
• W2735475571 hasConcept C192562407 @default.
• W2735475571 hasConcept C197336794 @default.
• W2735475571 hasConcept C2777529624 @default.
• W2735475571 hasConcept C2779732960 @default.
• W2735475571 hasConcept C33923547 @default.
• W2735475571 hasConcept C43617362 @default.
• W2735475571 hasConcept C55493867 @default.
• W2735475571 hasConcept C87023908 @default.
• W2735475571 hasConceptScore W2735475571C106847996 @default.
• W2735475571 hasConceptScore W2735475571C119128265 @default.
• W2735475571 hasConceptScore W2735475571C134306372 @default.
• W2735475571 hasConceptScore W2735475571C155672457 @default.
• W2735475571 hasConceptScore W2735475571C160756335 @default.
• W2735475571 hasConceptScore W2735475571C171250308 @default.
• W2735475571 hasConceptScore W2735475571C185592680 @default.
• W2735475571 hasConceptScore W2735475571C192562407 @default.
• W2735475571 hasConceptScore W2735475571C197336794 @default.
• W2735475571 hasConceptScore W2735475571C2777529624 @default.
• W2735475571 hasConceptScore W2735475571C2779732960 @default.
• W2735475571 hasConceptScore W2735475571C33923547 @default.
• W2735475571 hasConceptScore W2735475571C43617362 @default.
• W2735475571 hasConceptScore W2735475571C55493867 @default.
• W2735475571 hasConceptScore W2735475571C87023908 @default.

• next