Matches in SemOpenAlex for { <https://semopenalex.org/work/W2735708483> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2735708483 abstract "Casein micelle is a colloidal structure containing different casein molecules and calcium phosphate. Different forces are involved in this micellar organisation and contribute to their stability. However micellar destabilisations can be observed after modifications of physico-chemical conditions like acidification, calcium addition, intensive heat treatments, high pressure or proteolysis with as consequence the formation of a gel or a sediment. The objective of this work was to understand the physico-chemical modifications of casein micelles by AprX, an extracellular protease produced by a psychrotrophic bacteria Pseudomonas fluorescens commonly found in raw milk. For this, we studied the milk destabilisation at macroscopic, colloidal and molecular levels after 1. Inoculation of raw milk by Pseudomonas fluorescens and UHT treatment; 2. Addition of chromatographically purified AprX protease from Pseudomonas fluorescens in milk. For both types of experiment (addition of bacteria or enzyme), destabilisation appeared visually (presence of sediment) and progressively as a function of time. This destabilisation was also determined by measuring the phosphate stability named Ramsdell test. Aggregates were formed with in parallel decreases in the zeta potential and hydration of casein micelles. At molecular level, peptides were released from casein micelles. By reversed phase liquid chromatography coupled to tandem mass spectrometry, one part of the peptides was identified. The as1-, as2-, ab- and k-caseins were hydrolyzed with a quantitative preference for b-casein.The decrease in the stability of casein micelles will be discussed in relation with the modifications of structure and the observed proteolysis. Potential application of this research in term of detection of unstable UHT milks will be proposed knowing that this enzyme is heat-resistant and its implication in the destabilisation of UHT milk during its storage is often evoked." @default.
- W2735708483 created "2017-07-21" @default.
- W2735708483 creator A5019453735 @default.
- W2735708483 creator A5044554453 @default.
- W2735708483 creator A5044677210 @default.
- W2735708483 creator A5054272182 @default.
- W2735708483 creator A5058887661 @default.
- W2735708483 creator A5069967714 @default.
- W2735708483 creator A5073977322 @default.
- W2735708483 creator A5074794900 @default.
- W2735708483 creator A5077209761 @default.
- W2735708483 creator A5082691281 @default.
- W2735708483 creator A5084412189 @default.
- W2735708483 date "2012-05-14" @default.
- W2735708483 modified "2023-10-15" @default.
- W2735708483 title "Hydrolysis of Casein Micelles by AprX Enzym from Pseudomonas fluorescens Induces their Déstabilisation" @default.
- W2735708483 hasPublicationYear "2012" @default.
- W2735708483 type Work @default.
- W2735708483 sameAs 2735708483 @default.
- W2735708483 citedByCount "0" @default.
- W2735708483 crossrefType "proceedings-article" @default.
- W2735708483 hasAuthorship W2735708483A5019453735 @default.
- W2735708483 hasAuthorship W2735708483A5044554453 @default.
- W2735708483 hasAuthorship W2735708483A5044677210 @default.
- W2735708483 hasAuthorship W2735708483A5054272182 @default.
- W2735708483 hasAuthorship W2735708483A5058887661 @default.
- W2735708483 hasAuthorship W2735708483A5069967714 @default.
- W2735708483 hasAuthorship W2735708483A5073977322 @default.
- W2735708483 hasAuthorship W2735708483A5074794900 @default.
- W2735708483 hasAuthorship W2735708483A5077209761 @default.
- W2735708483 hasAuthorship W2735708483A5082691281 @default.
- W2735708483 hasAuthorship W2735708483A5084412189 @default.
- W2735708483 hasBestOaLocation W27357084831 @default.
- W2735708483 hasConcept C11268172 @default.
- W2735708483 hasConcept C178790620 @default.
- W2735708483 hasConcept C184651966 @default.
- W2735708483 hasConcept C185592680 @default.
- W2735708483 hasConcept C2776142715 @default.
- W2735708483 hasConcept C2776835041 @default.
- W2735708483 hasConcept C2781463711 @default.
- W2735708483 hasConcept C31903555 @default.
- W2735708483 hasConcept C43617362 @default.
- W2735708483 hasConcept C523546767 @default.
- W2735708483 hasConcept C54355233 @default.
- W2735708483 hasConcept C55493867 @default.
- W2735708483 hasConcept C86803240 @default.
- W2735708483 hasConcept C89423630 @default.
- W2735708483 hasConcept C94412978 @default.
- W2735708483 hasConceptScore W2735708483C11268172 @default.
- W2735708483 hasConceptScore W2735708483C178790620 @default.
- W2735708483 hasConceptScore W2735708483C184651966 @default.
- W2735708483 hasConceptScore W2735708483C185592680 @default.
- W2735708483 hasConceptScore W2735708483C2776142715 @default.
- W2735708483 hasConceptScore W2735708483C2776835041 @default.
- W2735708483 hasConceptScore W2735708483C2781463711 @default.
- W2735708483 hasConceptScore W2735708483C31903555 @default.
- W2735708483 hasConceptScore W2735708483C43617362 @default.
- W2735708483 hasConceptScore W2735708483C523546767 @default.
- W2735708483 hasConceptScore W2735708483C54355233 @default.
- W2735708483 hasConceptScore W2735708483C55493867 @default.
- W2735708483 hasConceptScore W2735708483C86803240 @default.
- W2735708483 hasConceptScore W2735708483C89423630 @default.
- W2735708483 hasConceptScore W2735708483C94412978 @default.
- W2735708483 hasLocation W27357084831 @default.
- W2735708483 hasOpenAccess W2735708483 @default.
- W2735708483 hasPrimaryLocation W27357084831 @default.
- W2735708483 hasRelatedWork W2045545154 @default.
- W2735708483 hasRelatedWork W2124561857 @default.
- W2735708483 hasRelatedWork W2164105024 @default.
- W2735708483 hasRelatedWork W2368263852 @default.
- W2735708483 hasRelatedWork W2370629835 @default.
- W2735708483 hasRelatedWork W2442432435 @default.
- W2735708483 hasRelatedWork W2499387634 @default.
- W2735708483 hasRelatedWork W2982396801 @default.
- W2735708483 hasRelatedWork W3036848024 @default.
- W2735708483 hasRelatedWork W4220819982 @default.
- W2735708483 isParatext "false" @default.
- W2735708483 isRetracted "false" @default.
- W2735708483 magId "2735708483" @default.
- W2735708483 workType "article" @default.