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- W2735721259 abstract "Abstract Visual read-out diagnostics tools are promising candidates for field applicable medical devices. Current colorimetric biosensors require introduction of natural enzymes or nanozymes, which has some serious drawbacks for practical applications. Chitosan, a natural polymer, provides safe and efficient compound in medical and pharmaceutical technology. Herein, we report on a simple, cost-efficient, field-portable, environmental friendly and ultra-sensitive multiplex detection platform based on peroxidase-like activity of chitosan in the presence of 3,3’,5,5’-Tetramethylbenzidine (TMBZ) and H 2 O 2 . This straight forward signal amplification strategy was successfully applied to detect H 2 O 2 , glucose and lactate with the limit of detection (LOD) of 2.64 pM, 0.104 μM and 2.8 nM respectively, represents the lowest LOD of H 2 O 2 , glucose and lactate with visual read-out. The chitosan-based assay performance was also retained in complex biological media for glucose and lactate detection. Furthermore, the proposed assay was successfully demonstrated as a paper-based colorimetric biosensor. Most importantly, the simplicity, biocompatibility and sensitivity of the proposed assay will open new doors for instrument free naked eye visual detection of H 2 O 2 , glucose and lactate detection." @default.
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- W2735721259 date "2017-07-12" @default.
- W2735721259 modified "2023-09-27" @default.
- W2735721259 title "Chitozyme: First Peroxidase-like Activity of Chitosan for Multiplexed Visual Detection of H2O2, Glucose and Lactate on Paper-based Device" @default.
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- W2735721259 doi "https://doi.org/10.1101/162446" @default.
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