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- W2736121336 abstract "Mycobacterium neoaurum is a saprophytic, soil-dwelling bacterium. The strain NRRL B-3805 converts phytosterols to androst-4-ene-3,17-dione (androstenedione; AD), a precursor of multiple C19 steroids of importance to industry. NRRL B-3805 itself is able to convert AD to other steroid products, including testosterone (Ts) and androst-1,4-diene-3,17-dione (androstadienedione; ADD). However to improve this strain for industrial use, genetic modification is a priority. In this chapter, we describe a range of genetic techniques that can be used for M. neoaurum NRRL B-3805. Methods for transformation, expression, and gene knockouts are presented as well as plasmid maintenance and stability." @default.
- W2736121336 created "2017-07-21" @default.
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- W2736121336 date "2017-01-01" @default.
- W2736121336 modified "2023-09-30" @default.
- W2736121336 title "Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacterium neoaurum NRRL B-3805" @default.
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- W2736121336 doi "https://doi.org/10.1007/978-1-4939-7183-1_7" @default.
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