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• W2736556066 abstract "HomePlant DiseaseVol. 101, No. 12First Association of Pedilanthus leaf curl virus, Papaya leaf curl virus, Cotton leaf curl Kokhran virus, and Papaya leaf curl betasatellite with Symptomatic Chilli Pepper in Pakistan PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Association of Pedilanthus leaf curl virus, Papaya leaf curl virus, Cotton leaf curl Kokhran virus, and Papaya leaf curl betasatellite with Symptomatic Chilli Pepper in PakistanS. Yasmin, N. I. Raja, S. Hameed, and J. K. BrownS. YasminSearch for more papers by this author, N. I. RajaSearch for more papers by this author, S. HameedSearch for more papers by this author, and J. K. Brown†Corresponding author: J. K. Brown; E-mail: E-mail Address: JBrown@ag.arizona.eduSearch for more papers by this authorAffiliationsAuthors and Affiliations S. Yasmin N. I. Raja , PMAS Arid Agriculture University, Rawalpindi, Pakistan S. Hameed , Crop Diseases Research Institute, National Agriculture Research Centre, Islamabad, Pakistan J. K. Brown † , School of Plant Sciences, University of Arizona, Tucson, AZ 85721. Published Online:29 Sep 2017https://doi.org/10.1094/PDIS-06-17-0883-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Chilli pepper is an important crop in Pakistan. Recently, chilli leaf curl symptoms have occurred in Islamabad with 10 to 50% disease incidence. During 2014, leaf curl symptoms reminiscent of begomovirus infection were observed in chilli pepper plants at experimental fields of the National Agriculture Research Centre, Islamabad. Leaves were collected from 12 symptomatic plants and analyzed by TAS-ELISA, using the monoclonal antibody panel of Harrison et al. (1997). Total DNA was purified from six plants found positive by TAS-ELISA, hereafter isolates 248 and 249, using the DNeasy Plant Mini-kit (QIAGEN). The possible presence of begomovirus was investigated using PCR amplification with degenerate primers (F-5′-GGRTTDGARGCATGHGTACANGCC-3′ and R-5′-GCCHATRTAYAGRAAGCCMAGRAT-3′). The expected 650 bp amplicons were cloned into the pGEM-T Easy plasmid vector and subjected to Sanger sequencing. BLASTn analysis indicated that isolate 248 showed 97% nucleotide (nt) sequence identity with Pedilanthus leaf curl virus (PeLCV) and 93% to Papaya leaf curl virus (PaLCuV) for isolate 249-1, while the isolate 249-2 shared 95% nt sequence identity with Cotton leaf curl Kokhran virus-Burewala (CLCuKoV-Bu). Rolling circle amplified (RCA) product for isolates 248 and 249 was digested with HindIII, EcoRI, and NcoI, ∼2.8-kb fragment was cloned in pGEM-3Z and subjected to bidirectional DNA sequencing using primer walking, with a minimum overlap of 150 bases. The cloned p248, p249-1, and p249-2 begomoviral genomes were 2,772, 2,772, and 2,782 nt in length. Pairwise nt comparisons using the Sequence Demarcation Tool v1.2 (Muhire et al. 2014) indicated that 248-ISD was closely related to PeLCV (AM712436.1) at 97% nt identity, and 249-1 shared 96% nt identity to PaLCuV-Rh (FM955601) reported to infect chilli (Vinoth Kumar et al. 2015), whereas 249-2 was 95% identical to CLCuKoV-Bu (LN845932.1). At ≥91% nt sequence identity, they are considered isolates of the previously reported species, CLCuKoV-Bu, PaLCuV-Rh, and PeLCV, respectively (Brown et al. 2015). The possible presence of begomovirus-associated betasatellite was investigated by PCR amplification using the primers (F-5′-GGTACCGCCGGAGCTTAGCWCKCC-3′ and R-5′-GGTACCGTAGCTAAGGCTGCTG-3′) and DNA sequencing of the cloned amplicon. BLASTn analysis (GenBank) identified the closest relative as an isolate of Papaya leaf curl virus betasatellite (PaLCuB) (JN663874) at 92% nt identity (SDT). This is the first report of PeLCV (KY978406), PaLCuV (KY97840), CLCuKoV (KY825244), and PaLCuB (KY825245) associated with chilli pepper plants in Pakistan. Severe yield losses associated with begomoviral infection of chilli pepper underscore the need for virus-resistant cultivars.References:Brown, J. K., et al. 2015. Arch. Virol. 160:1593. https://doi.org/10.1007/s00705-015-2398-y Crossref, ISI, Google ScholarHarrison, B. D., et al. 1997. Ann. Appl. Biol. 130:61. Google ScholarMuhire, B. M., et al. 2014. PLoS One 9:e108277. https://doi.org/10.1371/journal.pone.0108277 Crossref, ISI, Google ScholarVinoth Kumar, R., et al. 2015. J. Gen. Virol. 96:3143. https://doi.org/10.1099/jgv.0.000254 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 12 December 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 16 Nov 2017Published: 29 Sep 2017First Look: 25 Jul 2017Accepted: 20 Jul 2017 Page: 2155 Information© 2017 The American Phytopathological SocietyCited byLeaf Curl Disease a Significant Constraint in the Production of Tomato: Impact, Challenges, and Management14 December 2022Analysis of the effects of the mutation of selected genes of pedilanthus leaf curl virus on infectivity, symptoms and the maintenance of tobacco leaf curl betasatellite25 May 2022 | Canadian Journal of Plant Pathology, Vol. 44, No. 5Cotton leaf curl Kokhran virusCABI Compendium, Vol. CABI CompendiumPapaya leaf curl virusCABI Compendium, Vol. CABI CompendiumPedilanthus leaf curl virusCABI Compendium, Vol. CABI CompendiumPapaya leaf curl betasatelliteCABI Compendium, Vol. CABI CompendiumGenomic Designing for Breeding Biotic Stress Resistant Pepper Crop3 July 2022Association of Tomato leaf curl Karnataka virus and satellites with enation leaf curl disease of Pseuderanthemum reticulatum (Radlk.) a new ornamental host for begomovirus infecting tomato in India8 June 2021 | Indian Phytopathology, Vol. 74, No. 4Genus Capsicum L. and Diseases of Sweet and Hot Peppers (Review)15 October 2021 | Bulletin of Science and Practice, Vol. 7, No. 10Codiaeum variegatum in Pakistan harbours pedilanthus leaf curl virus and papaya leaf curl virus as well as a newly identified betasatellite23 May 2020 | Archives of Virology, Vol. 165, No. 8Begomovirus Menace and Its Management in Vegetable Crops24 March 2020Identification of two further agriculturally important begomoviruses and their associated satellites infecting the weed Digera arvensis in Pakistan14 June 2019 | European Journal of Plant Pathology, Vol. 155, No. 2Capsicum annuum and Capsicum frutescens (Bell pepper, Chilli, Pepper, Sweet pepper)6 June 2020Identification of pedilanthus leaf curl virus and associated betasatellite infecting turnip in Pakistan24 May 2018 | Journal of Plant Pathology, Vol. 100, No. 2First report of Tomato leaf curl virus and Croton yellow vein mosaic betasatellite infecting chilli plants in Pakistan27 February 2018 | New Disease Reports, Vol. 37, No. 1" @default.
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