Matches in SemOpenAlex for { <https://semopenalex.org/work/W2736628949> ?p ?o ?g. }
- W2736628949 endingPage "10" @default.
- W2736628949 startingPage "2" @default.
- W2736628949 abstract "The Arabidopsis thaliana gene encoding CYP71A16 is part of the gene cluster for the biosynthesis and modification of the triterpenoid marneral. Previous investigations of A. thaliana have revealed that CYP71A16 catalyzes marneral oxidation, while it also can accept marnerol as substrate. The aim of the present study was to investigate functional properties of CYP71A16 in vitro. For this purpose, heterologous expression of a N-terminally modified version of CYP71A16 was established in Escherichia coli, which yielded up to 50mgL-1 recombinant enzyme. The enzyme was purified and activity was reconstituted in vitro with different redox partners. A heterologous bacterial redox partner system consisting of the flavodoxin YkuN from Bacillus subtilis and the flavodoxin reductase Fpr from E. coli clearly outperformed the cytochrome P450 reductase ATR2 from A. thaliana in supporting the CYP71A16-mediated hydroxylation of marnerol. Substrate binding experiments with CYP71A16 revealed a dissociation constant KD of 225μM for marnerol. CYP71A16 catalyzed the hydroxylation of marnerol to 23-hydroxymarnerol with a KM of 142μM and a kcat of 3.9min-1. Furthermore, GC/MS analysis revealed an as of yet unidentified overoxidation product of this in vitro reaction. This article is part of a Special Issue entitled: Cytochrome P450 biodiversity and biotechnology, edited by Erika Plettner, Gianfranco Gilardi, Luet Wong, Vlada Urlacher, Jared Goldstone." @default.
- W2736628949 created "2017-07-31" @default.
- W2736628949 creator A5020529811 @default.
- W2736628949 creator A5022831554 @default.
- W2736628949 creator A5029516970 @default.
- W2736628949 creator A5031851274 @default.
- W2736628949 creator A5050050354 @default.
- W2736628949 creator A5056768547 @default.
- W2736628949 date "2018-01-01" @default.
- W2736628949 modified "2023-09-26" @default.
- W2736628949 title "Insights into the functional properties of the marneral oxidase CYP71A16 from Arabidopsis thaliana" @default.
- W2736628949 cites W1532212858 @default.
- W2736628949 cites W1791503532 @default.
- W2736628949 cites W1906542637 @default.
- W2736628949 cites W1929126126 @default.
- W2736628949 cites W1963970760 @default.
- W2736628949 cites W1964135958 @default.
- W2736628949 cites W1972558437 @default.
- W2736628949 cites W1982356218 @default.
- W2736628949 cites W1982857351 @default.
- W2736628949 cites W1985504443 @default.
- W2736628949 cites W1986482685 @default.
- W2736628949 cites W1987545492 @default.
- W2736628949 cites W1989253713 @default.
- W2736628949 cites W1990324427 @default.
- W2736628949 cites W1994744663 @default.
- W2736628949 cites W2001419953 @default.
- W2736628949 cites W2003236418 @default.
- W2736628949 cites W2003562022 @default.
- W2736628949 cites W2007253437 @default.
- W2736628949 cites W2012801837 @default.
- W2736628949 cites W2016649780 @default.
- W2736628949 cites W2018925791 @default.
- W2736628949 cites W2021842042 @default.
- W2736628949 cites W2025614596 @default.
- W2736628949 cites W2027406139 @default.
- W2736628949 cites W2034551014 @default.
- W2736628949 cites W2042228810 @default.
- W2736628949 cites W2042447251 @default.
- W2736628949 cites W2045073305 @default.
- W2736628949 cites W2052704239 @default.
- W2736628949 cites W2054375136 @default.
- W2736628949 cites W2055309018 @default.
- W2736628949 cites W2059423368 @default.
- W2736628949 cites W2070955854 @default.
- W2736628949 cites W2072592991 @default.
- W2736628949 cites W2074858254 @default.
- W2736628949 cites W2076943035 @default.
- W2736628949 cites W2101802925 @default.
- W2736628949 cites W2113271899 @default.
- W2736628949 cites W2114343994 @default.
- W2736628949 cites W2119180692 @default.
- W2736628949 cites W2125112299 @default.
- W2736628949 cites W2155909615 @default.
- W2736628949 cites W2170455232 @default.
- W2736628949 cites W2171240744 @default.
- W2736628949 cites W2294225971 @default.
- W2736628949 cites W2320337426 @default.
- W2736628949 cites W2321723590 @default.
- W2736628949 cites W2331086861 @default.
- W2736628949 cites W2516127834 @default.
- W2736628949 cites W2573242419 @default.
- W2736628949 cites W2586402208 @default.
- W2736628949 cites W4210360711 @default.
- W2736628949 cites W4293247451 @default.
- W2736628949 cites W859753841 @default.
- W2736628949 doi "https://doi.org/10.1016/j.bbapap.2017.07.008" @default.
- W2736628949 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28734978" @default.
- W2736628949 hasPublicationYear "2018" @default.
- W2736628949 type Work @default.
- W2736628949 sameAs 2736628949 @default.
- W2736628949 citedByCount "8" @default.
- W2736628949 countsByYear W27366289492018 @default.
- W2736628949 countsByYear W27366289492020 @default.
- W2736628949 countsByYear W27366289492021 @default.
- W2736628949 countsByYear W27366289492022 @default.
- W2736628949 countsByYear W27366289492023 @default.
- W2736628949 crossrefType "journal-article" @default.
- W2736628949 hasAuthorship W2736628949A5020529811 @default.
- W2736628949 hasAuthorship W2736628949A5022831554 @default.
- W2736628949 hasAuthorship W2736628949A5029516970 @default.
- W2736628949 hasAuthorship W2736628949A5031851274 @default.
- W2736628949 hasAuthorship W2736628949A5050050354 @default.
- W2736628949 hasAuthorship W2736628949A5056768547 @default.
- W2736628949 hasConcept C104317684 @default.
- W2736628949 hasConcept C108305142 @default.
- W2736628949 hasConcept C134651460 @default.
- W2736628949 hasConcept C143065580 @default.
- W2736628949 hasConcept C181199279 @default.
- W2736628949 hasConcept C185592680 @default.
- W2736628949 hasConcept C2775988993 @default.
- W2736628949 hasConcept C2777272437 @default.
- W2736628949 hasConcept C2779155592 @default.
- W2736628949 hasConcept C2781109383 @default.
- W2736628949 hasConcept C40767141 @default.
- W2736628949 hasConcept C41183919 @default.
- W2736628949 hasConcept C523546767 @default.
- W2736628949 hasConcept C54355233 @default.