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- W2736691376 abstract "Obesity and associated comorbidities such as cardiac diseases have become a major public health problem for today’s societies. There has been an increased focus on the use of natural compounds to combat this growing pandemic. Many studies have concluded that dietary polyphenolic flavonoids can reduce the development of mature adipocytes, a prerequisite for obesity, but little is known about the stages and mechanisms by which flavonoids act. Human preadipocytes (SGBS cells), a novel adipogenesis model, were treated with various concentrations of the flavonoid quercetin during proliferation and differentiation. Cell proliferation was measured using a 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT) assay after 72 hours of incubation. These were supported by microscopic cell counts. Differentiation was measured by oil red “O” staining followed by semi quantitative spectrophotometric analysis and lipid development examination. Quercetin was shown to reduce preadipocyte proliferation at high concentrations but increased viability at low concentrations. This has previously been reported in 3T3 cells. However, differentiation was reduced significantly across all concentrations of quercetin (p" @default.
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- W2736691376 date "2016-10-01" @default.
- W2736691376 modified "2023-09-26" @default.
- W2736691376 title "P39 Effects of quercetin on adipogenesis: a hope for obesity and associated comorbities" @default.
- W2736691376 doi "https://doi.org/10.1136/heartjnl-2016-310696.43" @default.
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