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- W2736707398 endingPage "275" @default.
- W2736707398 startingPage "268" @default.
- W2736707398 abstract "High frequency ultrasound backscatter signals from sea urchin oocytes were measured using a 40 MHz transducer and compared to numerical simulations. The Faran scattering model was used to calculate the ultrasound scattered from single oocytes in suspension. The urchin oocytes are non-nucleated with uniform size and biomechanical properties; the backscatter from each cell is similar and easy to simulate, unlike typical nucleated mammalian cells. The time domain signal measured from single oocytes in suspension showed two distinct peaks, and the power spectrum was periodic with minima spaced approximately 10 MHz apart. Good agreement to the Faran scattering model was observed. Measurements from tightly packed oocyte cell pellets showed similar periodic features in the power spectra, which was a result of the uniform size and consistent biomechanical properties of the cells. Numerical simulations that calculated the ultrasound scattered from individual oocytes within a three dimensional volume showed good agreement to the measured signals and B-scan images. A cepstral analysis of the signal was used to calculate the size of the cells, which was 78.7 μm (measured) and 81.4 μm (simulated). This work supports the single scattering approximation, where ultrasound is discretely scattered from single cells within a bulk homogeneous sample, and that multiple scattering has a negligible effect. This technique can be applied towards understanding the complex scattering behaviour from heterogeneous tissues." @default.
- W2736707398 created "2017-07-31" @default.
- W2736707398 creator A5027024186 @default.
- W2736707398 creator A5034907812 @default.
- W2736707398 creator A5068273693 @default.
- W2736707398 creator A5070669210 @default.
- W2736707398 date "2017-07-01" @default.
- W2736707398 modified "2023-09-26" @default.
- W2736707398 title "High frequency ultrasound imaging and simulations of sea urchin oocytes" @default.
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- W2736707398 doi "https://doi.org/10.1121/1.4993594" @default.
- W2736707398 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28764480" @default.