FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2737038670> ?p ?o ?g. }

• W2737038670 abstract "ABSTRACT Herpesvirus capsids assemble in the nucleus, while final virion maturation proceeds in the cytoplasm. This requires that newly formed nucleocapsids cross the nuclear envelope (NE), which occurs by budding at the inner nuclear membrane (INM), release of the primary enveloped virion into the perinuclear space (PNS), and subsequent rapid fusion with the outer nuclear membrane (ONM). During this process, the NE remains intact, even at late stages of infection. In addition, the spacing between the INM and ONM is maintained, as is that between the primary virion envelope and nuclear membranes. The linker of nucleoskeleton and cytoskeleton (LINC) complex consists of INM proteins with a luminal SUN (Sad1/UNC-84 homology) domain connected to ONM proteins with a KASH (Klarsicht, ANC-1, SYNE homology) domain and is thought to be responsible for spacing the nuclear membranes. To investigate the role of the LINC complex during herpesvirus infection, we generated cell lines constitutively expressing dominant negative (dn) forms of SUN1 and SUN2. Ultrastructural analyses revealed a significant expansion of the PNS and the contiguous intracytoplasmic lumen, most likely representing endoplasmic reticulum (ER), especially in cells expressing dn-SUN2. After infection, primary virions accumulated in these expanded luminal regions, also very distant from the nucleus. The importance of the LINC complex was also confirmed by reduced progeny virus titers in cells expressing dn-SUN2. These data show that the intact LINC complex is required for efficient nuclear egress of herpesviruses, likely acting to promote fusion of primary enveloped virions with the ONM. IMPORTANCE While the viral factors for primary envelopment of nucleocapsids at the inner nuclear membrane are known to the point of high-resolution structures, the roles of cellular components and regulators remain enigmatic. Furthermore, the machinery responsible for fusion with the outer nuclear membrane is unsolved. We show here that dominant negative SUN2 interferes with efficient herpesvirus nuclear egress, apparently by interfering with fusion between the primary virion envelope and outer nuclear membrane. This identifies a new cellular component important for viral egress and implicates LINC complex integrity in nonconventional nuclear membrane trafficking." @default.
• W2737038670 created "2017-07-31" @default.
• W2737038670 creator A5005165347 @default.
• W2737038670 creator A5005194409 @default.
• W2737038670 creator A5017599509 @default.
• W2737038670 creator A5040364929 @default.
• W2737038670 creator A5049949632 @default.
• W2737038670 creator A5063464132 @default.
• W2737038670 creator A5090871900 @default.
• W2737038670 date "2017-10-01" @default.
• W2737038670 modified "2023-10-16" @default.
• W2737038670 title "Integrity of the Linker of Nucleoskeleton and Cytoskeleton Is Required for Efficient Herpesvirus Nuclear Egress" @default.
• W2737038670 cites W1496271097 @default.
• W2737038670 cites W1805914897 @default.
• W2737038670 cites W1907539319 @default.
• W2737038670 cites W1985880387 @default.
• W2737038670 cites W1989538493 @default.
• W2737038670 cites W1993960918 @default.
• W2737038670 cites W1998724633 @default.
• W2737038670 cites W2005321454 @default.
• W2737038670 cites W2021304970 @default.
• W2737038670 cites W2022575121 @default.
• W2737038670 cites W2030276925 @default.
• W2737038670 cites W2035838778 @default.
• W2737038670 cites W2037933581 @default.
• W2737038670 cites W2070989134 @default.
• W2737038670 cites W2079671128 @default.
• W2737038670 cites W2080523738 @default.
• W2737038670 cites W2087144305 @default.
• W2737038670 cites W2091862729 @default.
• W2737038670 cites W2115407835 @default.
• W2737038670 cites W2117347549 @default.
• W2737038670 cites W2119349019 @default.
• W2737038670 cites W2120055283 @default.
• W2737038670 cites W2133119603 @default.
• W2737038670 cites W2138357022 @default.
• W2737038670 cites W2138395245 @default.
• W2737038670 cites W2140157503 @default.
• W2737038670 cites W2152963843 @default.
• W2737038670 cites W2160146556 @default.
• W2737038670 cites W2163504716 @default.
• W2737038670 cites W2165913063 @default.
• W2737038670 cites W2168480092 @default.
• W2737038670 cites W2230398306 @default.
• W2737038670 cites W2304517011 @default.
• W2737038670 cites W3048247788 @default.
• W2737038670 cites W4211058615 @default.
• W2737038670 cites W4366956078 @default.
• W2737038670 doi "https://doi.org/10.1128/jvi.00330-17" @default.
• W2737038670 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5599750" @default.
• W2737038670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28724767" @default.
• W2737038670 hasPublicationYear "2017" @default.
• W2737038670 type Work @default.
• W2737038670 sameAs 2737038670 @default.
• W2737038670 citedByCount "20" @default.
• W2737038670 countsByYear W27370386702017 @default.
• W2737038670 countsByYear W27370386702018 @default.
• W2737038670 countsByYear W27370386702019 @default.
• W2737038670 countsByYear W27370386702020 @default.
• W2737038670 countsByYear W27370386702021 @default.
• W2737038670 countsByYear W27370386702022 @default.
• W2737038670 countsByYear W27370386702023 @default.
• W2737038670 crossrefType "journal-article" @default.
• W2737038670 hasAuthorship W2737038670A5005165347 @default.
• W2737038670 hasAuthorship W2737038670A5005194409 @default.
• W2737038670 hasAuthorship W2737038670A5017599509 @default.
• W2737038670 hasAuthorship W2737038670A5040364929 @default.
• W2737038670 hasAuthorship W2737038670A5049949632 @default.
• W2737038670 hasAuthorship W2737038670A5063464132 @default.
• W2737038670 hasAuthorship W2737038670A5090871900 @default.
• W2737038670 hasBestOaLocation W27370386701 @default.
• W2737038670 hasConcept C103038307 @default.
• W2737038670 hasConcept C104317684 @default.
• W2737038670 hasConcept C126725582 @default.
• W2737038670 hasConcept C142669718 @default.
• W2737038670 hasConcept C1491633281 @default.
• W2737038670 hasConcept C158617107 @default.
• W2737038670 hasConcept C159047783 @default.
• W2737038670 hasConcept C190062978 @default.
• W2737038670 hasConcept C2522874641 @default.
• W2737038670 hasConcept C2777896290 @default.
• W2737038670 hasConcept C2780723820 @default.
• W2737038670 hasConcept C28859421 @default.
• W2737038670 hasConcept C29512474 @default.
• W2737038670 hasConcept C54355233 @default.
• W2737038670 hasConcept C65871279 @default.
• W2737038670 hasConcept C86339819 @default.
• W2737038670 hasConcept C86803240 @default.
• W2737038670 hasConcept C94912109 @default.
• W2737038670 hasConcept C95444343 @default.
• W2737038670 hasConcept C97380033 @default.
• W2737038670 hasConceptScore W2737038670C103038307 @default.
• W2737038670 hasConceptScore W2737038670C104317684 @default.
• W2737038670 hasConceptScore W2737038670C126725582 @default.
• W2737038670 hasConceptScore W2737038670C142669718 @default.
• W2737038670 hasConceptScore W2737038670C1491633281 @default.
• W2737038670 hasConceptScore W2737038670C158617107 @default.
• W2737038670 hasConceptScore W2737038670C159047783 @default.
• W2737038670 hasConceptScore W2737038670C190062978 @default.
• W2737038670 hasConceptScore W2737038670C2522874641 @default.

• next