Matches in SemOpenAlex for { <https://semopenalex.org/work/W2737645474> ?p ?o ?g. }
- W2737645474 endingPage "9546" @default.
- W2737645474 startingPage "9528" @default.
- W2737645474 abstract "A variety of diseases are caused by deficiencies in amounts or activity of key proteins. An approach that increases the amount of a specific protein might be of therapeutic benefit. We reasoned that translation could be specifically enhanced using trans-acting agents that counter the function of negative regulatory elements present in the 5' UTRs of some mRNAs. We recently showed that translation can be enhanced by antisense oligonucleotides (ASOs) that target upstream open reading frames. Here we report the amount of a protein can also be selectively increased using ASOs designed to hybridize to other translation inhibitory elements in 5' UTRs. Levels of human RNASEH1, LDLR, and ACP1 and of mouse ACP1 and ARF1 were increased up to 2.7-fold in different cell types and species upon treatment with chemically modified ASOs targeting 5' UTR inhibitory regions in the mRNAs encoding these proteins. The activities of ASOs in enhancing translation were sequence and position dependent and required helicase activity. The ASOs appear to improve the recruitment of translation initiation factors to the target mRNA. Importantly, ASOs targeting ACP1 mRNA significantly increased the level of ACP1 protein in mice, suggesting that this approach has therapeutic and research potentials." @default.
- W2737645474 created "2017-07-31" @default.
- W2737645474 creator A5035551086 @default.
- W2737645474 creator A5041988426 @default.
- W2737645474 creator A5045676896 @default.
- W2737645474 creator A5050043536 @default.
- W2737645474 creator A5054926078 @default.
- W2737645474 creator A5062772468 @default.
- W2737645474 creator A5074034944 @default.
- W2737645474 creator A5076329672 @default.
- W2737645474 creator A5077755519 @default.
- W2737645474 creator A5084968340 @default.
- W2737645474 creator A5086530950 @default.
- W2737645474 creator A5090099741 @default.
- W2737645474 date "2017-07-21" @default.
- W2737645474 modified "2023-10-16" @default.
- W2737645474 title "Antisense oligonucleotides targeting translation inhibitory elements in 5′ UTRs can selectively increase protein levels" @default.
- W2737645474 cites W1510555818 @default.
- W2737645474 cites W1510897788 @default.
- W2737645474 cites W1534178113 @default.
- W2737645474 cites W1546570611 @default.
- W2737645474 cites W1546686895 @default.
- W2737645474 cites W1602545070 @default.
- W2737645474 cites W1752221293 @default.
- W2737645474 cites W1885175685 @default.
- W2737645474 cites W1910618147 @default.
- W2737645474 cites W1913136372 @default.
- W2737645474 cites W1964813963 @default.
- W2737645474 cites W1976104012 @default.
- W2737645474 cites W1982980697 @default.
- W2737645474 cites W1984832802 @default.
- W2737645474 cites W1985013843 @default.
- W2737645474 cites W1985567838 @default.
- W2737645474 cites W1988595759 @default.
- W2737645474 cites W1997689650 @default.
- W2737645474 cites W2000137108 @default.
- W2737645474 cites W2001314633 @default.
- W2737645474 cites W2002664542 @default.
- W2737645474 cites W2003993220 @default.
- W2737645474 cites W2008410413 @default.
- W2737645474 cites W2010511706 @default.
- W2737645474 cites W2012893162 @default.
- W2737645474 cites W2016299657 @default.
- W2737645474 cites W2017047779 @default.
- W2737645474 cites W2020469175 @default.
- W2737645474 cites W2021809694 @default.
- W2737645474 cites W2024751967 @default.
- W2737645474 cites W2025722577 @default.
- W2737645474 cites W2026022524 @default.
- W2737645474 cites W2033425827 @default.
- W2737645474 cites W2037313242 @default.
- W2737645474 cites W2039742014 @default.
- W2737645474 cites W2041450354 @default.
- W2737645474 cites W2043986644 @default.
- W2737645474 cites W2045868610 @default.
- W2737645474 cites W2056673221 @default.
- W2737645474 cites W2058970225 @default.
- W2737645474 cites W2061092005 @default.
- W2737645474 cites W2062299199 @default.
- W2737645474 cites W2066796446 @default.
- W2737645474 cites W2072971665 @default.
- W2737645474 cites W2076642805 @default.
- W2737645474 cites W2081224211 @default.
- W2737645474 cites W2081306620 @default.
- W2737645474 cites W2087064069 @default.
- W2737645474 cites W2119336718 @default.
- W2737645474 cites W2120624230 @default.
- W2737645474 cites W2129718981 @default.
- W2737645474 cites W2130218870 @default.
- W2737645474 cites W2130886644 @default.
- W2737645474 cites W2139553455 @default.
- W2737645474 cites W2141157874 @default.
- W2737645474 cites W2142951254 @default.
- W2737645474 cites W2145445573 @default.
- W2737645474 cites W2145502099 @default.
- W2737645474 cites W2152052780 @default.
- W2737645474 cites W2152974197 @default.
- W2737645474 cites W2154031143 @default.
- W2737645474 cites W2155456813 @default.
- W2737645474 cites W2158855280 @default.
- W2737645474 cites W2164220348 @default.
- W2737645474 cites W2164754533 @default.
- W2737645474 cites W2168765510 @default.
- W2737645474 cites W2170009139 @default.
- W2737645474 cites W2173732482 @default.
- W2737645474 cites W2261840374 @default.
- W2737645474 cites W2263984188 @default.
- W2737645474 cites W2282922301 @default.
- W2737645474 cites W2427010553 @default.
- W2737645474 cites W2464724577 @default.
- W2737645474 cites W2508930612 @default.
- W2737645474 cites W4237588029 @default.
- W2737645474 cites W4247599887 @default.
- W2737645474 cites W4253489635 @default.
- W2737645474 cites W4253808924 @default.
- W2737645474 cites W4256140813 @default.
- W2737645474 doi "https://doi.org/10.1093/nar/gkx632" @default.
- W2737645474 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5766168" @default.