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• W2737721824 abstract "Abstract Expanding the substrate scope of enzymes opens up new routes for synthesis of valuable chemicals. Ketone‐functionalized fatty acid derivatives and corresponding chiral alcohols are valuable building blocks for the synthesis of a variety of chemicals including pharmaceuticals. The alcohol dehydrogenase from Candida parapsilosis (cpADH5) catalyzes the reversible oxidations of chiral alcohols and has a broad substrate range; a challenge for cpADH5 is to convert alcohols with small substituents (methyl or ethyl) next to the oxidized alcohol moiety. Molecular docking studies revealed that W286 is located in the small binding pocket and limits the access to substrates that contain aliphatic chains longer than ethyl substituent. In the current manuscript, we report that positions L119 and W286 are key residues to boost oxidation of medium chain methyl 3‐hydroxy fatty acids; interestingly the enantiopreference toward methyl 3‐hydroxybutyrate was inverted. Kinetic characterization of W286A showed a 5.5 fold increase of V max and a 9.6 fold decrease of K m values toward methyl 3‐hydroxyhexanoate ( V max : 2.48 U mg − and K m : 4.76 m m ). Simultaneous saturation at positions 119 and 286 library yielded a double mutant (L119M/W286S) with more than 30‐fold improved activity toward methyl 3‐hydroxyoctanoate (WT: no conversion; L119M/W286S: 30 %) and inverted enantiopreference ( S ‐enantiomer ≥99 % activity decrease and R ‐enantiomer >20‐fold activity improvement) toward methyl 3‐hydroxybutyrate." @default.
• W2737721824 created "2017-07-31" @default.
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• W2737721824 date "2017-08-09" @default.
• W2737721824 modified "2023-10-16" @default.
• W2737721824 title "Inversion of cpADH5 Enantiopreference and Altered Chain Length Specificity for Methyl 3-Hydroxyalkanoates" @default.
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• W2737721824 doi "https://doi.org/10.1002/chem.201702581" @default.
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