Matches in SemOpenAlex for { <https://semopenalex.org/work/W2738203605> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2738203605 endingPage "599" @default.
- W2738203605 startingPage "594" @default.
- W2738203605 abstract "The fabrication of artificial luciferases (ALucs) with unique optical properties has a fundamental impact on bioassays and molecular imaging. In this study, we developed a new lineage of ALucs with unique substrate preferences by extracting consensus amino acids from the alignment of 25 copepod luciferase sequences available in natural luciferase pools. The primary sequence was first created with a sequence logo generator resulting in a total of 11 sibling sequences. Phylogenetic analysis shows that the newly fabricated ALucs form an independent branch, genetically isolated from the natural luciferases, and from a prior series of ALucs produced by our laboratory using a smaller basis set. The new lineage of ALucs were strongly luminescent in living mammalian cells with specific substrate selectivity to native coelenterazine. A single-residue-level comparison of the C-terminal sequences of new ALucs reveals that some amino acids in the C-terminal ends are greatly influential on the optical intensities but limited in the color variance. The success of this approach guides on how to engineer and functionalize marine luciferases for bioluminescence imaging and assays." @default.
- W2738203605 created "2017-07-31" @default.
- W2738203605 creator A5026459010 @default.
- W2738203605 creator A5033571299 @default.
- W2738203605 creator A5035143437 @default.
- W2738203605 creator A5056509156 @default.
- W2738203605 date "2017-08-09" @default.
- W2738203605 modified "2023-10-15" @default.
- W2738203605 title "Fabrication of a New Lineage of Artificial Luciferases from Natural Luciferase Pools" @default.
- W2738203605 cites W1591311438 @default.
- W2738203605 cites W1970877840 @default.
- W2738203605 cites W1972028384 @default.
- W2738203605 cites W1990715554 @default.
- W2738203605 cites W2021298125 @default.
- W2738203605 cites W2068176347 @default.
- W2738203605 cites W2098956821 @default.
- W2738203605 cites W2125214592 @default.
- W2738203605 cites W2134774350 @default.
- W2738203605 cites W2145049033 @default.
- W2738203605 cites W2158266834 @default.
- W2738203605 cites W2158569304 @default.
- W2738203605 cites W2167300757 @default.
- W2738203605 cites W2172711071 @default.
- W2738203605 cites W2324790354 @default.
- W2738203605 cites W2328859984 @default.
- W2738203605 cites W2470844118 @default.
- W2738203605 doi "https://doi.org/10.1021/acscombsci.7b00081" @default.
- W2738203605 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28742969" @default.
- W2738203605 hasPublicationYear "2017" @default.
- W2738203605 type Work @default.
- W2738203605 sameAs 2738203605 @default.
- W2738203605 citedByCount "17" @default.
- W2738203605 countsByYear W27382036052018 @default.
- W2738203605 countsByYear W27382036052019 @default.
- W2738203605 countsByYear W27382036052020 @default.
- W2738203605 countsByYear W27382036052021 @default.
- W2738203605 countsByYear W27382036052022 @default.
- W2738203605 countsByYear W27382036052023 @default.
- W2738203605 crossrefType "journal-article" @default.
- W2738203605 hasAuthorship W2738203605A5026459010 @default.
- W2738203605 hasAuthorship W2738203605A5033571299 @default.
- W2738203605 hasAuthorship W2738203605A5035143437 @default.
- W2738203605 hasAuthorship W2738203605A5056509156 @default.
- W2738203605 hasConcept C104317684 @default.
- W2738203605 hasConcept C111335760 @default.
- W2738203605 hasConcept C185592680 @default.
- W2738203605 hasConcept C18903297 @default.
- W2738203605 hasConcept C193252679 @default.
- W2738203605 hasConcept C2776817793 @default.
- W2738203605 hasConcept C2777289219 @default.
- W2738203605 hasConcept C2781309008 @default.
- W2738203605 hasConcept C2909536089 @default.
- W2738203605 hasConcept C5176359 @default.
- W2738203605 hasConcept C54009773 @default.
- W2738203605 hasConcept C55493867 @default.
- W2738203605 hasConcept C70721500 @default.
- W2738203605 hasConcept C86803240 @default.
- W2738203605 hasConceptScore W2738203605C104317684 @default.
- W2738203605 hasConceptScore W2738203605C111335760 @default.
- W2738203605 hasConceptScore W2738203605C185592680 @default.
- W2738203605 hasConceptScore W2738203605C18903297 @default.
- W2738203605 hasConceptScore W2738203605C193252679 @default.
- W2738203605 hasConceptScore W2738203605C2776817793 @default.
- W2738203605 hasConceptScore W2738203605C2777289219 @default.
- W2738203605 hasConceptScore W2738203605C2781309008 @default.
- W2738203605 hasConceptScore W2738203605C2909536089 @default.
- W2738203605 hasConceptScore W2738203605C5176359 @default.
- W2738203605 hasConceptScore W2738203605C54009773 @default.
- W2738203605 hasConceptScore W2738203605C55493867 @default.
- W2738203605 hasConceptScore W2738203605C70721500 @default.
- W2738203605 hasConceptScore W2738203605C86803240 @default.
- W2738203605 hasFunder F4320334764 @default.
- W2738203605 hasIssue "9" @default.
- W2738203605 hasLocation W27382036051 @default.
- W2738203605 hasLocation W27382036052 @default.
- W2738203605 hasOpenAccess W2738203605 @default.
- W2738203605 hasPrimaryLocation W27382036051 @default.
- W2738203605 hasRelatedWork W1511281879 @default.
- W2738203605 hasRelatedWork W2151818001 @default.
- W2738203605 hasRelatedWork W2157338212 @default.
- W2738203605 hasRelatedWork W2300469513 @default.
- W2738203605 hasRelatedWork W2784490748 @default.
- W2738203605 hasRelatedWork W2792099120 @default.
- W2738203605 hasRelatedWork W2800687731 @default.
- W2738203605 hasRelatedWork W2950031322 @default.
- W2738203605 hasRelatedWork W3021775193 @default.
- W2738203605 hasRelatedWork W3189631099 @default.
- W2738203605 hasVolume "19" @default.
- W2738203605 isParatext "false" @default.
- W2738203605 isRetracted "false" @default.
- W2738203605 magId "2738203605" @default.
- W2738203605 workType "article" @default.