FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2738920936> ?p ?o ?g. }

• W2738920936 abstract "The Passiflora genus comprises hundreds of wild and cultivated species of passion fruit used for food, industrial, ornamental and medicinal purposes. Efforts to develop genomic tools for genetic analysis of P. edulis, the most important commercial Passiflora species, are still incipient. In spite of many recognized applications of microsatellite markers in genetics and breeding, their availability for passion fruit research remains restricted. Microsatellite markers in P. edulis are usually limited in number, show reduced polymorphism, and are mostly based on compound or imperfect repeats. Furthermore, they are confined to only a few Passiflora species. We describe the use of NGS technology to partially assemble the P. edulis genome in order to develop hundreds of new microsatellite markers.A total of 14.11 Gbp of Illumina paired-end sequence reads were analyzed to detect simple sequence repeat sites in the sour passion fruit genome. A sample of 1300 contigs containing perfect repeat microsatellite sequences was selected for PCR primer development. Panels of di- and tri-nucleotide repeat markers were then tested in P. edulis germplasm accessions for validation. DNA polymorphism was detected in 74% of the markers (PIC = 0.16 to 0.77; number of alleles/locus = 2 to 7). A core panel of highly polymorphic markers (PIC = 0.46 to 0.77) was used to cross-amplify PCR products in 79 species of Passiflora (including P. edulis), belonging to four subgenera (Astrophea, Decaloba, Distephana and Passiflora). Approximately 71% of the marker/species combinations resulted in positive amplicons in all species tested. DNA polymorphism was detected in germplasm accessions of six closely related Passiflora species (P. edulis, P. alata, P. maliformis, P. nitida, P. quadrangularis and P. setacea) and the data used for accession discrimination and species assignment.A database of P. edulis DNA sequences obtained by NGS technology was examined to identify microsatellite repeats in the sour passion fruit genome. Markers were submitted to evaluation using accessions of cultivated and wild Passiflora species. The new microsatellite markers detected high levels of DNA polymorphism in sour passion fruit and can potentially be used in genetic analysis of P. edulis and other Passiflora species." @default.
• W2738920936 created "2017-07-31" @default.
• W2738920936 creator A5033667073 @default.
• W2738920936 creator A5041082635 @default.
• W2738920936 creator A5047076234 @default.
• W2738920936 creator A5061004869 @default.
• W2738920936 creator A5075589016 @default.
• W2738920936 creator A5079587299 @default.
• W2738920936 date "2017-07-21" @default.
• W2738920936 modified "2023-10-14" @default.
• W2738920936 title "Microsatellite marker development by partial sequencing of the sour passion fruit genome (Passiflora edulis Sims)" @default.
• W2738920936 cites W134153613 @default.
• W2738920936 cites W1595601536 @default.
• W2738920936 cites W1748758095 @default.
• W2738920936 cites W178751556 @default.
• W2738920936 cites W1899104497 @default.
• W2738920936 cites W1952015772 @default.
• W2738920936 cites W1969746417 @default.
• W2738920936 cites W1972586190 @default.
• W2738920936 cites W1975805818 @default.
• W2738920936 cites W1976688131 @default.
• W2738920936 cites W1990319111 @default.
• W2738920936 cites W1992856682 @default.
• W2738920936 cites W1994233487 @default.
• W2738920936 cites W2002741418 @default.
• W2738920936 cites W2008112905 @default.
• W2738920936 cites W2012204109 @default.
• W2738920936 cites W2021367706 @default.
• W2738920936 cites W2028861416 @default.
• W2738920936 cites W2032105503 @default.
• W2738920936 cites W2036056816 @default.
• W2738920936 cites W2043396048 @default.
• W2738920936 cites W2045565479 @default.
• W2738920936 cites W2045938698 @default.
• W2738920936 cites W2052284079 @default.
• W2738920936 cites W2062400071 @default.
• W2738920936 cites W2070722344 @default.
• W2738920936 cites W2072575028 @default.
• W2738920936 cites W2081597100 @default.
• W2738920936 cites W2081632 @default.
• W2738920936 cites W2082974611 @default.
• W2738920936 cites W2098126593 @default.
• W2738920936 cites W2103571367 @default.
• W2738920936 cites W2103740237 @default.
• W2738920936 cites W2104557145 @default.
• W2738920936 cites W2111008358 @default.
• W2738920936 cites W2114795390 @default.
• W2738920936 cites W2115246163 @default.
• W2738920936 cites W2121546023 @default.
• W2738920936 cites W2123351065 @default.
• W2738920936 cites W2123437298 @default.
• W2738920936 cites W2123829497 @default.
• W2738920936 cites W2124131884 @default.
• W2738920936 cites W2124188192 @default.
• W2738920936 cites W2125827783 @default.
• W2738920936 cites W2125909173 @default.
• W2738920936 cites W2131107966 @default.
• W2738920936 cites W2134739162 @default.
• W2738920936 cites W2139760555 @default.
• W2738920936 cites W2140373717 @default.
• W2738920936 cites W2160969485 @default.
• W2738920936 cites W2161339576 @default.
• W2738920936 cites W2162348455 @default.
• W2738920936 cites W2165205212 @default.
• W2738920936 cites W2166466493 @default.
• W2738920936 cites W2167084861 @default.
• W2738920936 cites W2167553418 @default.
• W2738920936 cites W2175314143 @default.
• W2738920936 cites W2182859959 @default.
• W2738920936 cites W4242641176 @default.
• W2738920936 doi "https://doi.org/10.1186/s12864-017-3881-5" @default.
• W2738920936 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5520361" @default.
• W2738920936 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28732469" @default.
• W2738920936 hasPublicationYear "2017" @default.
• W2738920936 type Work @default.
• W2738920936 sameAs 2738920936 @default.
• W2738920936 citedByCount "27" @default.
• W2738920936 countsByYear W27389209362018 @default.
• W2738920936 countsByYear W27389209362019 @default.
• W2738920936 countsByYear W27389209362020 @default.
• W2738920936 countsByYear W27389209362021 @default.
• W2738920936 countsByYear W27389209362022 @default.
• W2738920936 countsByYear W27389209362023 @default.
• W2738920936 crossrefType "journal-article" @default.
• W2738920936 hasAuthorship W2738920936A5033667073 @default.
• W2738920936 hasAuthorship W2738920936A5041082635 @default.
• W2738920936 hasAuthorship W2738920936A5047076234 @default.
• W2738920936 hasAuthorship W2738920936A5061004869 @default.
• W2738920936 hasAuthorship W2738920936A5075589016 @default.
• W2738920936 hasAuthorship W2738920936A5079587299 @default.
• W2738920936 hasBestOaLocation W27389209361 @default.
• W2738920936 hasConcept C104317684 @default.
• W2738920936 hasConcept C141231307 @default.
• W2738920936 hasConcept C144024400 @default.
• W2738920936 hasConcept C149923435 @default.
• W2738920936 hasConcept C180754005 @default.
• W2738920936 hasConcept C190789776 @default.
• W2738920936 hasConcept C2777461220 @default.
• W2738920936 hasConcept C2779510327 @default.
• W2738920936 hasConcept C2908647359 @default.

• next