Matches in SemOpenAlex for { <https://semopenalex.org/work/W2738961369> ?p ?o ?g. }
- W2738961369 endingPage "2165" @default.
- W2738961369 startingPage "2155" @default.
- W2738961369 abstract "The fabrication of cell sheets is a major requirement for bottom-up tissue engineering purposes (e.g., cell sheet engineering) and regenerative medicine. Employing thermoresponsive polymer coatings as tissue culture substrates allows for the mild, temperature-triggered detachment of intact cell sheets along with their extracellular matrix (ECM). It has been shown before that biocompatible, thermoresponsive poly(glycidyl ether) monolayers on gold substrates can be utilized to harvest confluent cell sheets by simply reducing the temperature to 20 °C. Herein, we report on the first poly(glycidyl ether)-based coating on an application-relevant tissue culture plastic substrate. We devised a simple, substrate-geometry-independent method to functionalize polystyrene (PS) surfaces from dilute ethanolic solution via the physical adsorption process of a thermoresponsive poly(glycidyl ether) block copolymer (PGE) bearing a short, hydrophobic, and photoreactive benzophenone (BP) anchor block. Subsequently, the PGE-coated PS is UV-irradiated for covalent photoimmobilization of the polymer on the PS substrate. Online monitoring of the adsorption process via QCM-D measurements and detailed characterization of the resulting coatings via AFM, ellipsometry, and water contact angle (CA) measurements revealed the formation of an ultrathin PGE layer with an average dry thickness of 0.7 ± 0.1 nm. Adhesion and proliferation of human dermal fibroblasts on PGE-coated PS and tissue culture PS (TCPS) were comparable. For temperature-triggered detachment, fibroblasts were cultured in PGE-coated PS culture dishes at 37 °C for 24 h until they reached confluency. Intact cell sheets could be harvested from the thermoresponsive substrates within 51 ± 17 min upon cooling to 20 °C, whereas sheets could not be harvested from uncoated PS and TCPS control dishes. Live/dead staining and flow cytometry affirmed a high viability of the fibroblasts within the cell sheets. Hence, ultrathin layers of thermoresponsive poly(glycidyl ether)s on hydrophobic PS substrates are functional coatings for cell sheet fabrication." @default.
- W2738961369 created "2017-07-31" @default.
- W2738961369 creator A5021478960 @default.
- W2738961369 creator A5030662388 @default.
- W2738961369 creator A5037819369 @default.
- W2738961369 creator A5066407873 @default.
- W2738961369 creator A5077702330 @default.
- W2738961369 date "2017-08-01" @default.
- W2738961369 modified "2023-10-18" @default.
- W2738961369 title "Ultrathin Poly(glycidyl ether) Coatings on Polystyrene for Temperature-Triggered Human Dermal Fibroblast Sheet Fabrication" @default.
- W2738961369 cites W1554555407 @default.
- W2738961369 cites W1605481523 @default.
- W2738961369 cites W1964453076 @default.
- W2738961369 cites W1965630124 @default.
- W2738961369 cites W1969552041 @default.
- W2738961369 cites W1973943743 @default.
- W2738961369 cites W1975471033 @default.
- W2738961369 cites W1975479312 @default.
- W2738961369 cites W1980604115 @default.
- W2738961369 cites W1982761616 @default.
- W2738961369 cites W1985826950 @default.
- W2738961369 cites W1986503147 @default.
- W2738961369 cites W1992615890 @default.
- W2738961369 cites W1996670544 @default.
- W2738961369 cites W1998435441 @default.
- W2738961369 cites W1999369954 @default.
- W2738961369 cites W2000385095 @default.
- W2738961369 cites W2003393269 @default.
- W2738961369 cites W2005148643 @default.
- W2738961369 cites W2005869545 @default.
- W2738961369 cites W2008870349 @default.
- W2738961369 cites W2011231339 @default.
- W2738961369 cites W2017480916 @default.
- W2738961369 cites W2020669057 @default.
- W2738961369 cites W2021951914 @default.
- W2738961369 cites W2028582430 @default.
- W2738961369 cites W2031028793 @default.
- W2738961369 cites W2033392724 @default.
- W2738961369 cites W2035017383 @default.
- W2738961369 cites W2035514216 @default.
- W2738961369 cites W2038460713 @default.
- W2738961369 cites W2041052342 @default.
- W2738961369 cites W2041460916 @default.
- W2738961369 cites W2042881776 @default.
- W2738961369 cites W2046680995 @default.
- W2738961369 cites W2063797951 @default.
- W2738961369 cites W2064027952 @default.
- W2738961369 cites W2070005779 @default.
- W2738961369 cites W2074558968 @default.
- W2738961369 cites W2075770220 @default.
- W2738961369 cites W2076913495 @default.
- W2738961369 cites W2077779617 @default.
- W2738961369 cites W2085596409 @default.
- W2738961369 cites W2086671635 @default.
- W2738961369 cites W2086841672 @default.
- W2738961369 cites W2089423454 @default.
- W2738961369 cites W2090370847 @default.
- W2738961369 cites W2107494332 @default.
- W2738961369 cites W2113102617 @default.
- W2738961369 cites W2116446546 @default.
- W2738961369 cites W2121263864 @default.
- W2738961369 cites W2123630792 @default.
- W2738961369 cites W2127415038 @default.
- W2738961369 cites W2138569000 @default.
- W2738961369 cites W2142372316 @default.
- W2738961369 cites W2165823862 @default.
- W2738961369 cites W2218337915 @default.
- W2738961369 cites W2229744996 @default.
- W2738961369 cites W2299229349 @default.
- W2738961369 cites W2312914754 @default.
- W2738961369 cites W2318611248 @default.
- W2738961369 cites W2478673073 @default.
- W2738961369 cites W2509119686 @default.
- W2738961369 cites W2511853919 @default.
- W2738961369 cites W2564500992 @default.
- W2738961369 cites W2635045778 @default.
- W2738961369 cites W837226679 @default.
- W2738961369 cites W961198422 @default.
- W2738961369 cites W2318917615 @default.
- W2738961369 doi "https://doi.org/10.1021/acsbiomaterials.7b00270" @default.
- W2738961369 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33440564" @default.
- W2738961369 hasPublicationYear "2017" @default.
- W2738961369 type Work @default.
- W2738961369 sameAs 2738961369 @default.
- W2738961369 citedByCount "13" @default.
- W2738961369 countsByYear W27389613692018 @default.
- W2738961369 countsByYear W27389613692020 @default.
- W2738961369 countsByYear W27389613692021 @default.
- W2738961369 countsByYear W27389613692022 @default.
- W2738961369 crossrefType "journal-article" @default.
- W2738961369 hasAuthorship W2738961369A5021478960 @default.
- W2738961369 hasAuthorship W2738961369A5030662388 @default.
- W2738961369 hasAuthorship W2738961369A5037819369 @default.
- W2738961369 hasAuthorship W2738961369A5066407873 @default.
- W2738961369 hasAuthorship W2738961369A5077702330 @default.
- W2738961369 hasConcept C111368507 @default.
- W2738961369 hasConcept C127313418 @default.
- W2738961369 hasConcept C127413603 @default.