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• W2739042478 abstract "INTRODUCTION : Skin and soft tissue infection (SSTIs) are common type of infection that may contribute to longer hospital stays, significantly increase the cost of medical care, and are likely to have an important role in the development of antimicrobial resistance. Most of these infections are superficial and readily treated with a regimen of local care and antibiotics. However, soft tissue infections involving deeper layers like fascia and muscle can rapidly progress to systemic sepsis and prove fatal.95 Staphylococcus aureus is one of the common organisms associated with soft tissue infection. S. aureus are ubiquitous organisms and among the most commonly encountered in medical practice. S. aureus has been reported as a major cause of community and hospital acquired infections. The organisms has a differential ability to spread and cause outbreaks in hospitals. Infections caused by S. aureus used to respond to β-lactam group of antibiotics. Penicillin – resistant S. aureus strains began emerging shortly after introduction of penicillin in 1940. Resistance to methicillin and other β-lactamase – resistant Penicillin was first observed in S.aureus soon after methicillin was introduced into clinical use in 1961. Hence this study has been undertaken to compare the conventional methods of detecting MRSA infection with the molecular technology and also to determine the antimicrobial susceptibility pattern of MRSA in a tertiary care hospital which will facilitate in the implementation of appropriate treatment of the patients.AIMS AND OBJECTIVES : 1) To detect MRSA strains from patients with soft tissue infection. 2) To compare the phenotypic and the genotypic identification methods in the detection of MRSA for their sensitivity, specificityand accuracy. 3) To compare the antimicrobial susceptibility pattern of MRSA and MSSA isolates. 4) To detect susceptibility pattern of the isolates to vancomycin by disc diffusion method and E-test. MATERIALS AND METHODS : This cross sectional study was carried out from January 2007–January 2008. Ethical and research clearance was obtained from the Institute of Ethical Committee Government General Hospital and Madras Medical College,Chennai. Permission to conduct the study was sought from the respective hospital authorities. Informed consent was obtained from the patients before enrolment into the study. Statistical analyses were carried out using Statistical Package for Social Sciences (SPSS) and Epi – info softwares. Pus samples were collected from skin and soft tissue infections from patients admitted in general surgery and allied specialities atGovt. General Hospital, Chennai. The collected pus samples were subjected to direct Gram stain and inoculated onto nutrient agar, blood agar and Mac Conkey agar. The plates are incubated at 37oC and inspected after overnight incubation. S. aureus was identified by its colony morphology , Gram stain and catalase test. Further confirmation was done by slide and tube coagulase test, growth on mannitol salt agar and DNase test by standardmicrobiological techniques as recommended by CLSI guidelines. RESULTS : Methicillin resistance was determined for a total of 150 S. aureus isolates from pus samples by oxacillin and cefoxitin disc diffusion method, oxacillin screen agar, MIC – broth micro dilution method, and PCR for mec A gene. Vancomycin resistance was determined by disc diffusion and E–test method. The antimicrobial susceptibility pattern to various groups of antibiotics were also done by Kirby Bauer disc diffusion method, The results are as follows: 95.6%strains were resistant to penicillin. Moderate level of resistance were seen to ampicillin,co-trimoxazole and cephalexin. However majority of strains were sensitive tocefotaxime, erythromycin, chloramphenicol ciprofloxacin, ofloxacin and rifampicin. All strains were sensitive to vancomycin,teicoplanin and linezolid. CONCLUSION : Among the Staphylococcus aureus isolated from patients with soft tissue infections, 54% were methicillin resistant. The conventional phenotypic methods in the detection of MRSA such as cefoxitin disc diffusion method, MIC – micro broth dilution method and oxacillin screen agar had a high degree of sensitivity and specificity when compared with that of mec A - the gold standard and hence it can be used in the detection of MRSA isolates. MRSA isolates were more resistant to many of the antimicrobials when compared with MSSAisolates. All the isolates were sensitive to vancomycin." @default.
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• W2739042478 date "2017-06-10" @default.
• W2739042478 modified "2023-09-25" @default.
• W2739042478 title "Detection of Methicillin Resistant Strains of Staphylococcus aureus Using Phenotypic and Genotypic Methods in a Tertiary Care Hospital" @default.
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• W2739042478 doi "https://doi.org/10.20546/ijcmas.2017.607.415" @default.
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