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- W2739347815 abstract "Four Fusarium oxysporum isolates, f.sp lycopersici Race 1 (Kis-1), Race 2 (880621a-1), Race 3 (Tomino-1c) and f.sp radicis lycopersici (MAFF 103044) were obtained from the stock of the Laboratory of Plant Pathology, Tokyo University of Agriculture and Technology, Japan. They were cultured on Potato Sucrose Agar (PSA) at 26.3°C and genomic DNAs were successfully extracted using a novel non -Phenol- Chloroform DNA extraction method. The DNA extracts were efficient as templates for polymerase chain reaction analysis using primer sets based on the Fusarium ribosomal DNA intergenic spacer region (IGS), as well as race-specific primers sp13 and sp23 primers, based on the endo- and exo-polygalacturonase gene sequences. The results showed that the method recently described for the extraction of DNA from Magnaporthe grisea could also be used for fusarial DNA extraction useful in widespread diagnostics and molecular analyses." @default.
- W2739347815 created "2017-07-31" @default.
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- W2739347815 date "2007-01-01" @default.
- W2739347815 modified "2023-09-24" @default.
- W2739347815 title "Evaluation of the Efficiency of the Products of a Non-Phenol-Chloroform Dna Extraction Method as Templates for Pcr Analysis of Some Fusarium oxysporum Isolates" @default.
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