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• W2739699995 abstract "Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV infection represents a challenge. Currently there is no vaccine against HDV. Recombinant production of HDV antigen (HDAg) is the first step towards a potential vaccine candidate and the development of assays for HDV detection. This study demonstrates the expression of one HDAg isoform, S-HDAg, in Pichia pastoris. A recombinant vector carrying a tagged gene encoding S-HDAg under the control of the methanol-inducible promoter AOX1 was designed and integrated into P. pastoris X33. The protein, which was purified using a Ni2+ affinity column and eluted at 100–150 mM imidazole, has potential as a recombinant antigen for further study." @default.
• W2739699995 created "2017-08-08" @default.
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• W2739699995 date "2017-07-27" @default.
• W2739699995 modified "2023-10-07" @default.
• W2739699995 title "Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris" @default.
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• W2739699995 doi "https://doi.org/10.1186/s13104-017-2692-8" @default.
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