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- W2740197796 abstract "ABSTRACT A method for isolation of coupled mitochondria isolated from the liver of adult Nile tilapia Oreochromis niloticus is described for the first time. They were metabolically active, able to sustain oxidative phosphorylation, as shown by respiratory control ratio values, which were about 5.8±0.8 and 3.3±0.4 when respiring on malate/piruvate (complex I) or succinate (complex II), respectively, as substrate. Membrane potential exhibited a value of approximately 197±4mV for malate/piruvate or succinate. The procedure now described for the isolation of O. niloticus mitochondria is an important new tool, allowing the study about the effect of xenobiotics on mitochondrial bioenergetic, being evaluated the effect of oxyfluorfen (0.6mgL -1 ) in the liver mitocondrial bioenergetic. These results showed that phosphorylation was significantly affected by oxyfluorfen which contributed to the decrease on the liver cell energy charge and consequently led to the fish dead. Keywords: tilapia, Oreochromis niloticus, oxyfluorfen, mitochondria, liver" @default.
- W2740197796 created "2017-08-08" @default.
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- W2740197796 date "2009-01-01" @default.
- W2740197796 modified "2023-09-27" @default.
- W2740197796 title "Caracterização da mitocôndria isolada de fígado de tilápia-do-nilo (Oreochromis niloticus) e alterações da bioenergética mitocondrial causadas pela exposição ao herbicida oxifluorfena (Characterization of liver mitochondria from Nile tilapia (Oreochromis niloticus) and mitochondrial bioenergetics alterations caused by exposure to oxyfluorfen herbicide)" @default.
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