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- W2740241199 abstract "Summary. A series of mutants of E. coli temperature-sensitive for DNA synthesis has been studied. The temperature-sensitive DNA mutations map in seven distinct genetic loci most of which have not been previously reported. Mutations in dnaA and in dnaC affect the initiation of DNA replication; those at the remaining loci affect chain elongation. A temperature-sensitive F'lae is shown to suppress a group A mutant with somewhat less efficiency than other F' factors previously reported by others. The gene products rendered temperaturesensitive by the mutations have not been identified for any of the loci. This report attempts to analyze the complexity of normal DNA replication in E. coli by defining a number of genetic loci whose products are involved in replication. Previous work has defined three such loci (Hirota, Ryter and Jacob, 1968; Carl, 1970). Of 501 temperature-sens itive mutants isolated without applying any selective pressure, fifteen were found to be specifically affected in DNA replication. These mutants will be designated Dna-. We have mapped the mutations in fourteen of these, as well as in a number of strains isolated by other workers. The mutations occur at seven distinct locations on the E. coli chromosome." @default.
- W2740241199 created "2017-08-08" @default.
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- W2740241199 date "1971-01-01" @default.
- W2740241199 modified "2023-09-27" @default.
- W2740241199 title "Escherichia coli Mutants Temperature-Sensitive for DNA Synthesis" @default.
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