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- W2740288730 abstract "Themutation sof-i suppresses thesporulation defect ofmutations ineither thespoOB, spoOE, orspoOF stage 0sporulation genes. Through theuseofintegrative plasmids carrying theportion ofthechromosome including thespoOA locus andflanking regions, thesof-l mutation waslocalized nearthespoOA locus. Aplasmid carrying afragment ofDNAwithsofgenetic activity wasconstructed. Nucleic acidsequence analysis ofthis fragment revealed asingle basechange that resulted inasubstitution oflysine forasparagine inthe12thcodonofthe spoOA gene. Theresults indicate that certain missense mutations inthespoOA genebypass thenecessity forthe spoOB, spoOE, andspoOFgeneproducts insporulation. Several models fortheinteraction ofthese gene products maybeimagined. Theinitiation ofsporulation inBacillus subtilis maybe blocked bymutations atseveral genes called spoOgenes (9). Inaddition topreventing theformation oftheasymmetric septum, theinitial eventinsporulation, mutants carrying mutations inthese geneshaveawidevariety ofpleiotropic phenotypes (4). Ithasrecently beendiscovered that transcription fromseveral promoters controlled bysigma-37 or sigma-28 RNA polymerases isblocked byspoOmutants. Thusthetranscription ofthespoVGgene, agenetranscribed withsigma-32- orsigma-37-containing RNA polymerases, is blocked byspoOA, spoOB, andspoOE, spoOF, andspoOH mutations (14). Similarly, transcription oftwosigma-28 RNA polymerase-dependent genesisblocked byspoOA," @default.
- W2740288730 created "2017-08-08" @default.
- W2740288730 creator A5063549626 @default.
- W2740288730 date "1985-01-01" @default.
- W2740288730 modified "2023-09-23" @default.
- W2740288730 title "Identification oftheTranscriptional Suppressor sof-1 asan Alteration inthespoOAProteint" @default.
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- W2740288730 hasPublicationYear "1985" @default.
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