FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2740367159> ?p ?o ?g. }

• W2740367159 endingPage "512" @default.
• W2740367159 startingPage "499" @default.
• W2740367159 abstract "Alpha7 nicotinic acetylcholine receptors (α7 nAChRs) are important drug targets in neurological disorders and inflammation, making their detection and localization by validated antibodies highly desirable. However, tests in knockout animals raised questions about specificity of antibodies to mouse α7 nAChRs. To date, methods for validating antibodies for rat or human α7 nAChR have not been reported. We developed a gel-shift assay for western blots using GH4C1 cells expressing either native rat receptors or α7 nAChR-green fluorescent protein (GFP) chimeras to evaluate seven commercially available α7 nAChR antibodies. Blots with anti-GFP antibody detected GFP or α7 nAChR-GFP expressed in GH4C1 cells, and 125I-α-bungarotoxin binding and RNA analysis demonstrated α7 nAChR expression. Validated samples were used to evaluate α7 nAChR antibodies by western blot and immunofluorescence studies. These methods confirmed that two of seven α7 nAChR antibodies identify gel-shifts for α7 nAChR/nAChR-GFP but only one antibody demonstrated low background and significant immunofluorescence differences between wild-type and α7 nAChR expressing GH4C1 cells. However, that polyclonal antibody displayed lot-to-lot variability. Our findings suggest that careful validation methods are required for all α7 nAChR receptor species and antibody lots and that the gel-shift assay may allow for relatively rapid antibody screening." @default.
• W2740367159 created "2017-08-08" @default.
• W2740367159 creator A5011980264 @default.
• W2740367159 creator A5030975808 @default.
• W2740367159 date "2017-08-01" @default.
• W2740367159 modified "2023-09-26" @default.
• W2740367159 title "Evaluating Commercially Available Antibodies for Rat α7 Nicotinic Acetylcholine Receptors" @default.
• W2740367159 cites W1496436965 @default.
• W2740367159 cites W1570361834 @default.
• W2740367159 cites W1592986776 @default.
• W2740367159 cites W1617737713 @default.
• W2740367159 cites W1963979286 @default.
• W2740367159 cites W1965549069 @default.
• W2740367159 cites W1968523639 @default.
• W2740367159 cites W1969473874 @default.
• W2740367159 cites W1972620632 @default.
• W2740367159 cites W1976768935 @default.
• W2740367159 cites W1980917285 @default.
• W2740367159 cites W1982171918 @default.
• W2740367159 cites W1985491990 @default.
• W2740367159 cites W1990794090 @default.
• W2740367159 cites W2010646554 @default.
• W2740367159 cites W2017721215 @default.
• W2740367159 cites W2029187954 @default.
• W2740367159 cites W2033259485 @default.
• W2740367159 cites W2046103542 @default.
• W2740367159 cites W2054936592 @default.
• W2740367159 cites W2055595940 @default.
• W2740367159 cites W2070264071 @default.
• W2740367159 cites W2073295417 @default.
• W2740367159 cites W2081817431 @default.
• W2740367159 cites W2089150909 @default.
• W2740367159 cites W2115460562 @default.
• W2740367159 cites W2123846033 @default.
• W2740367159 cites W2171191165 @default.
• W2740367159 cites W2242777763 @default.
• W2740367159 cites W2296810948 @default.
• W2740367159 cites W2507306980 @default.
• W2740367159 cites W4240422451 @default.
• W2740367159 cites W570865489 @default.
• W2740367159 cites W962937402 @default.
• W2740367159 doi "https://doi.org/10.1369/0022155417725304" @default.
• W2740367159 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5582671" @default.
• W2740367159 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28763248" @default.
• W2740367159 hasPublicationYear "2017" @default.
• W2740367159 type Work @default.
• W2740367159 sameAs 2740367159 @default.
• W2740367159 citedByCount "18" @default.
• W2740367159 countsByYear W27403671592018 @default.
• W2740367159 countsByYear W27403671592019 @default.
• W2740367159 countsByYear W27403671592020 @default.
• W2740367159 countsByYear W27403671592021 @default.
• W2740367159 countsByYear W27403671592022 @default.
• W2740367159 crossrefType "journal-article" @default.
• W2740367159 hasAuthorship W2740367159A5011980264 @default.
• W2740367159 hasAuthorship W2740367159A5030975808 @default.
• W2740367159 hasBestOaLocation W27403671591 @default.
• W2740367159 hasConcept C104317684 @default.
• W2740367159 hasConcept C142613039 @default.
• W2740367159 hasConcept C153911025 @default.
• W2740367159 hasConcept C159654299 @default.
• W2740367159 hasConcept C170493617 @default.
• W2740367159 hasConcept C185592680 @default.
• W2740367159 hasConcept C188987157 @default.
• W2740367159 hasConcept C203014093 @default.
• W2740367159 hasConcept C2776415932 @default.
• W2740367159 hasConcept C2778236600 @default.
• W2740367159 hasConcept C2779570518 @default.
• W2740367159 hasConcept C55493867 @default.
• W2740367159 hasConcept C80161118 @default.
• W2740367159 hasConcept C85265743 @default.
• W2740367159 hasConcept C86803240 @default.
• W2740367159 hasConcept C956191 @default.
• W2740367159 hasConceptScore W2740367159C104317684 @default.
• W2740367159 hasConceptScore W2740367159C142613039 @default.
• W2740367159 hasConceptScore W2740367159C153911025 @default.
• W2740367159 hasConceptScore W2740367159C159654299 @default.
• W2740367159 hasConceptScore W2740367159C170493617 @default.
• W2740367159 hasConceptScore W2740367159C185592680 @default.
• W2740367159 hasConceptScore W2740367159C188987157 @default.
• W2740367159 hasConceptScore W2740367159C203014093 @default.
• W2740367159 hasConceptScore W2740367159C2776415932 @default.
• W2740367159 hasConceptScore W2740367159C2778236600 @default.
• W2740367159 hasConceptScore W2740367159C2779570518 @default.
• W2740367159 hasConceptScore W2740367159C55493867 @default.
• W2740367159 hasConceptScore W2740367159C80161118 @default.
• W2740367159 hasConceptScore W2740367159C85265743 @default.
• W2740367159 hasConceptScore W2740367159C86803240 @default.
• W2740367159 hasConceptScore W2740367159C956191 @default.
• W2740367159 hasIssue "9" @default.
• W2740367159 hasLocation W27403671591 @default.
• W2740367159 hasLocation W27403671592 @default.
• W2740367159 hasLocation W27403671593 @default.
• W2740367159 hasLocation W27403671594 @default.
• W2740367159 hasOpenAccess W2740367159 @default.
• W2740367159 hasPrimaryLocation W27403671591 @default.
• W2740367159 hasRelatedWork W1757489651 @default.
• W2740367159 hasRelatedWork W1973079678 @default.

• next