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- W2740437029 abstract "We havegenerated transgenic micethatexpressa diverserepertoire of human sequence immunoglobulins. Theexpression ofthis repertoire isdirected by lightand heavychainminilocus transgenes comprised ofhumanprotein coding sequencesinan unrearranged, germ-line configuration. Inthis paperwe describe theconstruction oftheseminiloci andthe composition oftheCDR3repertoire generated bythe transgenic mice.Thelargest transgene discussed isa heavychainminilocus thatincludes humanAand yl coding sequencestogether withtheir respective switch regions. Itconsists ofa single 61kbDNAfragment propagated inabacterial plasmid vector. Bothhuman heavychain classes areexpressed inanimals that carry thetransgene. Inlight chaintransgenic animals the unrearranged minilocus sequencesrecombine toform VJ joints thatuse allfivehumanJXsegments, resulting ina diversity ofhuman-like CDR3regions. Similarly, inheavychaintransgenics theinserted sequencesundergo VDJjoining complete withNregion addition togenerate ahuman-like VHCDR3repertoire. AllsixhumanJHsegments andatleast eight oftheten transgene encoded humanDsegments areexpressed. The transgenic animalsdescribed inthispaper represent a potential sourceofhumansequence antibodies forinvivotherapeutic applications." @default.
- W2740437029 created "2017-08-08" @default.
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- W2740437029 date "1992-01-01" @default.
- W2740437029 modified "2023-09-28" @default.
- W2740437029 title "Atransgenic mouse that expressesa diversity ofhuman sequenceheavyandlight chain immunoglobulins" @default.
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