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- W2740486539 abstract "Previous cytochemical studies on the distribution of aminopeptidase A (APA) in the lower digestive tract of laboratory rodents have shown that this exopeptidase is localized in the brush border and Golgi apparatus of enterocytes in the small intestine, capillary endothelial cells of the mucous, submucous and muscle layers and in enteroendocrine cells of adult laboratory rodents and, in addition, in lysosomes and the inframicrovillous membrane system of ileal and jejunal enterocytes of suckling rats and mice (Lojda & Gossrau, 1980; Gossrau, 1981; Gossrau ef al., 1984, Lojda et al., 1984). This paper reports new APA-positive structures in the muscle layer of the stomach, small intestine and colon of developing and adult rats and mice. Originally, the positive elements were considered to be only capillaries (Gossrau, 1981). Colchicine-treated and untreated male and female pregnant and non-pregnant Wistar rats and NMRI mice and suckling animals bred in our laboratory were decapitated under ether anaesthesia. Tissues were removed and quenched in liquid nitrogen. APA was localized in fresh cryostat sections treated with acetone-chloroform using ~-Glu-4methoxy-2-naphthylamide (MNA) as substrate, Fast Blue B or hexazotized New Fuchsin as the simultaneous coupler, and calcium ions as activator as described by Lojda & Gossrau (1980). In addition, alkaline phosphatase, aminopeptidase M (APM), dipeptidyl peptidase IV and acetylcholine esterase were investigated by single or double incubation with the methods of choice as given by Lojda et al. (1979) with and without counterstaining with Haematoxylin or Methylene Blue. Catecholamines were localized with the formaldehyde-induced fluorescence (FIF) procedure in freeze-dried, xylelemounted cryostat sections (Heene, 1968). The specificity of the APA reaction was confirmed by adding EDTA to the incubation medium and by omitting CaC12. Information on the physiological role of APA in the muscle layer was obtained by incubating with media containing equimolar concentrations of angiotensin II or bradykinin in the presence of the synthetic substrate a-Glu-MNA. Fig. 1 shows APA activity is situated between the circular and longitudinal smooth muscle layers in the rat colon and in a network-like system in the circular layer. The reaction is inhibited by EDTA and is weaker in the absence of calcium ions, indicating that the enzyme actually is APA. Angiotensin II and bradykinin inhibit the enzyme in the" @default.
- W2740486539 created "2017-08-08" @default.
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- W2740486539 date "1985-01-01" @default.
- W2740486539 modified "2023-09-27" @default.
- W2740486539 title "Aminopeptidase A the rat and mouse in the muscular layer of lower digestive tract" @default.
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