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- W2740695211 abstract "BACKGROUND: Multiplex polymerase chain reaction al lows amplification of multiple target sequences of a ge nome under identical conditions in a single tube. This “one shot” polymerase chain reaction detection is time and cost effective when large or multiple genes, with many target fragments are investigated. This is applicable for retino blastoma susceptibility gene having 27 exons with recur rent mutations reported at most of the 12 CGA codons. MATERIALS AND METHODS: Multiplex polymerase chain reaction assay for the amplification of 12 CGA codons, which constitutes about 50 % of retinoblastoma suscepti bility gene mutations has been designed. The time and cost (includes only reagent cost) involved in both multiplex and uniplex polymerase chain reaction was also calculated. RESULTS: Twelve CGA codons were multiplexed in 5 in stead of 12 uniplex polymerase chain reactions, which took 36 hours and 9.78 US$ whereas multiplex polymerase chain reaction took 15 hours and 6.88 US$. Multiplex poly merase chain reaction method saved 58.3% of time and 29.6% of cost over uniplex polymerase chain reaction. CONCLUSION: Saving time by more than half and cost by nearly a third would help clinicians and geneticists while counseling retinoblastoma patients." @default.
- W2740695211 created "2017-08-08" @default.
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- W2740695211 date "2006-01-01" @default.
- W2740695211 modified "2023-09-27" @default.
- W2740695211 title "Original Communication CGA codons multiplex PCR in rapid diagnosis of retinoblastoma" @default.
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