FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2740975387> ?p ?o ?g. }

• W2740975387 abstract "Transaminases have become a key tool in biocatalysis to introduce the amine functionality into a range of molecules like prochiral α-ketoacids and ketones. However, due to the necessity of shifting the equilibrium towards the product side (depending on the amine donor) an efficient amination system may require three enzymes. So far, this well-established transformation has mainly been performed in vitro by assembling all biocatalysts individually, which comes along with elaborate and costly preparation steps. We present the design and characterization of a flexible approach enabling a quick set-up of single-cell biocatalysts producing the desired enzymes. By choosing an appropriate co-expression strategy, a modular system was obtained, allowing for flexible plug-and-play combination of enzymes chosen from the toolbox of available transaminases and/or recycling enzymes tailored for the desired application. By using a two-plasmid strategy for the recycling enzyme and the transaminase together with chromosomal integration of an amino acid dehydrogenase, two enzyme modules could individually be selected and combined with specifically tailored E. coli strains. Various plug-and-play combinations of the enzymes led to the construction of a series of single-cell catalysts suitable for the amination of various types of substrates. On the one hand the fermentative amination of α-ketoacids coupled both with metabolic and non-metabolic cofactor regeneration was studied, giving access to the corresponding α-amino acids in up to 96% conversion. On the other hand, biocatalysts were employed in a non-metabolic, “in vitro-type” asymmetric reductive amination of the prochiral ketone 4-phenyl-2-butanone, yielding the amine in good conversion (77%) and excellent stereoselectivity (ee = 98%). The described modularized concept enables the construction of tailored single-cell catalysts which provide all required enzymes for asymmetric reductive amination in a flexible fashion, representing a more efficient approach for the production of chiral amines and amino acids." @default.
• W2740975387 created "2017-08-08" @default.
• W2740975387 creator A5009412615 @default.
• W2740975387 creator A5011297283 @default.
• W2740975387 creator A5078319936 @default.
• W2740975387 creator A5091235713 @default.
• W2740975387 creator A5091393671 @default.
• W2740975387 date "2017-07-28" @default.
• W2740975387 modified "2023-10-14" @default.
• W2740975387 title "In vivo plug-and-play: a modular multi-enzyme single-cell catalyst for the asymmetric amination of ketoacids and ketones" @default.
• W2740975387 cites W1869265908 @default.
• W2740975387 cites W1926603859 @default.
• W2740975387 cites W1971439989 @default.
• W2740975387 cites W1972177294 @default.
• W2740975387 cites W1974491154 @default.
• W2740975387 cites W1989488173 @default.
• W2740975387 cites W1990776258 @default.
• W2740975387 cites W1992683699 @default.
• W2740975387 cites W1994533881 @default.
• W2740975387 cites W1996629685 @default.
• W2740975387 cites W1999151261 @default.
• W2740975387 cites W2000810737 @default.
• W2740975387 cites W2003099237 @default.
• W2740975387 cites W2014117083 @default.
• W2740975387 cites W2015793616 @default.
• W2740975387 cites W2018998390 @default.
• W2740975387 cites W2019796789 @default.
• W2740975387 cites W2019897836 @default.
• W2740975387 cites W2031671287 @default.
• W2740975387 cites W2033556576 @default.
• W2740975387 cites W2036557444 @default.
• W2740975387 cites W2040851590 @default.
• W2740975387 cites W2046203583 @default.
• W2740975387 cites W2065335949 @default.
• W2740975387 cites W2067452122 @default.
• W2740975387 cites W2072659655 @default.
• W2740975387 cites W2074749327 @default.
• W2740975387 cites W2075501152 @default.
• W2740975387 cites W2076167006 @default.
• W2740975387 cites W2077437218 @default.
• W2740975387 cites W2093518771 @default.
• W2740975387 cites W2099250676 @default.
• W2740975387 cites W2107677212 @default.
• W2740975387 cites W2114182624 @default.
• W2740975387 cites W2116137883 @default.
• W2740975387 cites W2121197874 @default.
• W2740975387 cites W2122962387 @default.
• W2740975387 cites W2124374902 @default.
• W2740975387 cites W2125866757 @default.
• W2740975387 cites W2127597805 @default.
• W2740975387 cites W2127771414 @default.
• W2740975387 cites W2128635872 @default.
• W2740975387 cites W2128920785 @default.
• W2740975387 cites W2132417094 @default.
• W2740975387 cites W2148324898 @default.
• W2740975387 cites W2160084442 @default.
• W2740975387 cites W2160868951 @default.
• W2740975387 cites W2165847039 @default.
• W2740975387 cites W2199708805 @default.
• W2740975387 cites W2209619530 @default.
• W2740975387 cites W2314220527 @default.
• W2740975387 cites W2316076552 @default.
• W2740975387 cites W2319391002 @default.
• W2740975387 cites W2330299898 @default.
• W2740975387 cites W2424655797 @default.
• W2740975387 cites W2426604790 @default.
• W2740975387 cites W2470087878 @default.
• W2740975387 cites W2507799663 @default.
• W2740975387 cites W2524638452 @default.
• W2740975387 cites W2529963675 @default.
• W2740975387 cites W2564521442 @default.
• W2740975387 cites W2566462605 @default.
• W2740975387 cites W2592799985 @default.
• W2740975387 cites W4293247451 @default.
• W2740975387 cites W1977342009 @default.
• W2740975387 doi "https://doi.org/10.1186/s12934-017-0750-5" @default.
• W2740975387 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5534079" @default.
• W2740975387 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28754115" @default.
• W2740975387 hasPublicationYear "2017" @default.
• W2740975387 type Work @default.
• W2740975387 sameAs 2740975387 @default.
• W2740975387 citedByCount "10" @default.
• W2740975387 countsByYear W27409753872017 @default.
• W2740975387 countsByYear W27409753872018 @default.
• W2740975387 countsByYear W27409753872019 @default.
• W2740975387 countsByYear W27409753872020 @default.
• W2740975387 countsByYear W27409753872021 @default.
• W2740975387 countsByYear W27409753872022 @default.
• W2740975387 crossrefType "journal-article" @default.
• W2740975387 hasAuthorship W2740975387A5009412615 @default.
• W2740975387 hasAuthorship W2740975387A5011297283 @default.
• W2740975387 hasAuthorship W2740975387A5078319936 @default.
• W2740975387 hasAuthorship W2740975387A5091235713 @default.
• W2740975387 hasAuthorship W2740975387A5091393671 @default.
• W2740975387 hasBestOaLocation W27409753871 @default.
• W2740975387 hasConcept C104317684 @default.
• W2740975387 hasConcept C131779359 @default.
• W2740975387 hasConcept C154881586 @default.
• W2740975387 hasConcept C161790260 @default.
• W2740975387 hasConcept C178790620 @default.

• next