FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2740996984> ?p ?o ?g. }

• W2740996984 abstract "Background: Lung cancer stem cells are considered to be responsible for lung cancer progression and metastasis. However, little is known about how they actually promote aggressive behaviors of lung cancer. Methods: We transduced OCT3/4, SOX2, and KLF4 (hereafter, OSK) into a KRAS-mutated (G12S) human lung adenocarcinoma cell line (A549) using retrovirus vectors. Dome-shaped colonies appeared in the OSK transduced A549 cells from 10 to 15 days after transduction. The colonies were picked up and sub-cultured. We named them OSK-A549-Colony cells. We evaluated cancer stem cell properties in the OSK-A549-Colony cells in terms of their chemo resistance, and sphere formation ability. We also assessed organoids constructing ability by co-culture with mesenchymal stem cells (MSCs) and human umbilical vein epithelial cells (HUVECs) on low attachment plates. To clarify the molecular mechanisms that determined the properties of the OSK-A549-Colony cells, we performed microarray analysis. We further analyzed the molecular mechanisms of organoids construction by OSK-A549-Colony cells and searched for a method to destroy them. Results: The induced OSK-A549-colony cells were significantly more resistant to cisplatin than the parental A549 cells. Sphere forming ability was enhanced in the OSK-A549-Colony cells. Co-culture with MSCs and HUVECs showed that only the OSK-A549-Colony cells were able to construct consolidated organoids in vitro that mimicked bona-fide human lung cancer tissues. We named them “lung cancer organoids”. We found that interleukin-6 (IL-6), which was the most differentially expressed gene in the OSK-A549-Colony cells by microarray analysis, facilitated the formation of lung cancer organoids via the conversion of MSCs into alpha-smooth muscle actin (αSMA)-positive cells. Surprisingly, the combination of anti-IL-6 antibody and cisplatin could destroy the lung cancer organoids, while cisplatin alone could not. Conclusion: By transducing defined factors, A549 cells acquired lung cancer stem cell like properties. Analysis of chemosensitivity using the lung cancer organoids showed that the combination of IL-6 blockade and cisplatin could be a novel therapy targeting lung cancer stem cells. Citation Format: Hiroyuki Ogawa, Michiyo Koyanagi-Aoi, Yoshimasa Maniwa, Takashi Aoi. Interleukin-6 blockade, a novel cancer stem cell targeted therapy, attenuates lung cancer tissue construction [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 3902. doi:10.1158/1538-7445.AM2017-3902" @default.
• W2740996984 created "2017-08-08" @default.
• W2740996984 creator A5046043650 @default.
• W2740996984 creator A5063237654 @default.
• W2740996984 creator A5080427739 @default.
• W2740996984 creator A5082268022 @default.
• W2740996984 date "2017-07-01" @default.
• W2740996984 modified "2023-09-27" @default.
• W2740996984 title "Abstract 3902: Interleukin-6 blockade, a novel cancer stem cell targeted therapy, attenuates lung cancer tissue construction" @default.
• W2740996984 doi "https://doi.org/10.1158/1538-7445.am2017-3902" @default.
• W2740996984 hasPublicationYear "2017" @default.
• W2740996984 type Work @default.
• W2740996984 sameAs 2740996984 @default.
• W2740996984 citedByCount "0" @default.
• W2740996984 crossrefType "proceedings-article" @default.
• W2740996984 hasAuthorship W2740996984A5046043650 @default.
• W2740996984 hasAuthorship W2740996984A5063237654 @default.
• W2740996984 hasAuthorship W2740996984A5080427739 @default.
• W2740996984 hasAuthorship W2740996984A5082268022 @default.
• W2740996984 hasBestOaLocation W27409969841 @default.
• W2740996984 hasConcept C104317684 @default.
• W2740996984 hasConcept C121608353 @default.
• W2740996984 hasConcept C142724271 @default.
• W2740996984 hasConcept C145103041 @default.
• W2740996984 hasConcept C203014093 @default.
• W2740996984 hasConcept C2776256026 @default.
• W2740996984 hasConcept C2777933648 @default.
• W2740996984 hasConcept C28328180 @default.
• W2740996984 hasConcept C502942594 @default.
• W2740996984 hasConcept C54355233 @default.
• W2740996984 hasConcept C55427017 @default.
• W2740996984 hasConcept C55493867 @default.
• W2740996984 hasConcept C71924100 @default.
• W2740996984 hasConcept C81729549 @default.
• W2740996984 hasConcept C86803240 @default.
• W2740996984 hasConcept C95444343 @default.
• W2740996984 hasConcept C96232424 @default.
• W2740996984 hasConceptScore W2740996984C104317684 @default.
• W2740996984 hasConceptScore W2740996984C121608353 @default.
• W2740996984 hasConceptScore W2740996984C142724271 @default.
• W2740996984 hasConceptScore W2740996984C145103041 @default.
• W2740996984 hasConceptScore W2740996984C203014093 @default.
• W2740996984 hasConceptScore W2740996984C2776256026 @default.
• W2740996984 hasConceptScore W2740996984C2777933648 @default.
• W2740996984 hasConceptScore W2740996984C28328180 @default.
• W2740996984 hasConceptScore W2740996984C502942594 @default.
• W2740996984 hasConceptScore W2740996984C54355233 @default.
• W2740996984 hasConceptScore W2740996984C55427017 @default.
• W2740996984 hasConceptScore W2740996984C55493867 @default.
• W2740996984 hasConceptScore W2740996984C71924100 @default.
• W2740996984 hasConceptScore W2740996984C81729549 @default.
• W2740996984 hasConceptScore W2740996984C86803240 @default.
• W2740996984 hasConceptScore W2740996984C95444343 @default.
• W2740996984 hasConceptScore W2740996984C96232424 @default.
• W2740996984 hasLocation W27409969841 @default.
• W2740996984 hasOpenAccess W2740996984 @default.
• W2740996984 hasPrimaryLocation W27409969841 @default.
• W2740996984 hasRelatedWork W1990678923 @default.
• W2740996984 hasRelatedWork W2004331554 @default.
• W2740996984 hasRelatedWork W2012802922 @default.
• W2740996984 hasRelatedWork W2025872085 @default.
• W2740996984 hasRelatedWork W2034982916 @default.
• W2740996984 hasRelatedWork W2136595037 @default.
• W2740996984 hasRelatedWork W2321372744 @default.
• W2740996984 hasRelatedWork W2333330507 @default.
• W2740996984 hasRelatedWork W2345194485 @default.
• W2740996984 hasRelatedWork W2382044612 @default.
• W2740996984 hasRelatedWork W2405339666 @default.
• W2740996984 hasRelatedWork W2493084260 @default.
• W2740996984 hasRelatedWork W2742051940 @default.
• W2740996984 hasRelatedWork W2755502262 @default.
• W2740996984 hasRelatedWork W2886196489 @default.
• W2740996984 hasRelatedWork W2901677405 @default.
• W2740996984 hasRelatedWork W2907000243 @default.
• W2740996984 hasRelatedWork W3180701972 @default.
• W2740996984 hasRelatedWork W574904037 @default.
• W2740996984 hasRelatedWork W2116224782 @default.
• W2740996984 isParatext "false" @default.
• W2740996984 isRetracted "false" @default.
• W2740996984 magId "2740996984" @default.
• W2740996984 workType "article" @default.