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- W2741128297 abstract "An existing histochemical method was adapted to a-particle autoradiography to provide a means of quantifying acetylcholinesterase inhibition at myoneu.ral junctions. The aparticles were emitted from polonium 210 which was present in the precipitate after staining. A technique was designed for counting areas of dense a-particle tracks on the autoradiograms which corresponded to sites of acetylcholinesterase activity. An experiment was performed which showed that the track production rate was consistent for several diaphragm sections when incubated under identical conditions with no inhibitor. Tissue sections of this type, representing zero inhibition, were used as controls for inhibitor studies. It was also demonstrated that the track production rate was constant during the different exposure times used in the inhibition study. Diisopropylfluorophosphate was used to evaluate the autoradiographic method of quantifying acetylcholinesterase inhibition. A plot of log inhibitor concentration against percentage inhibition resulted in a smooth sigmoidal curvewhich istypical of irreversible inhibitors." @default.
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- W2741128297 date "1969-01-01" @default.
- W2741128297 modified "2023-09-27" @default.
- W2741128297 title "THE INHIBITION OF ACETYLCHOLINESTERASE AT THE MYONEURAL ,JUXCTION" @default.
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