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- W2741246937 abstract "Partially hydrolyzed guar gum (PHGG), an important supplemental dietary fiber, has been used as food ingredient in many industries. In this study, a novel β-mannanase gene (RmMan5A) from Rhizomucor miehei was successfully expressed in Pichia pastoris and subjected for PHGG production. Enzyme activity of fermentation supernatant reached 85,200 U mL−1 after 168 h high cell density fermentation. The purified RmMan5A exhibited the highest enzyme activity at pH 7.0 and 65 °C. RmMan5A was then employed for guar gum hydrolysis and PHGG obtained demonstrated a weight-average molecular weight (Mw) of 2.5 × 104 Da. Total dietary fiber accounted 90.6% of PHGG and 24.9% (w/w) of PHGG were identified as manno-oligosaccharides with degree of polymerization < 7. PHGG was further fractionated (F1–F4) by gradual ethanol precipitation. PHGG F1 with an Mw value of 3.6 × 104 Da and a mannose/galactose (M/G) ratio of 1.47 was precipitated initially, followed by PHGG F2 and F3 which showed lower Mw and higher M/G ratio. According to the structure analysis, the distribution of α-d-galactose of PHGG F1 was compact and regular, and that of other fractions was more random. A suitable β-mannanase for PHGG production and some useful information of PHGG are provided in this paper." @default.
- W2741246937 created "2017-08-08" @default.
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- W2741246937 date "2017-12-01" @default.
- W2741246937 modified "2023-09-30" @default.
- W2741246937 title "High level expression of β-mannanase (RmMan5A) in Pichia pastoris for partially hydrolyzed guar gum production" @default.
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- W2741246937 doi "https://doi.org/10.1016/j.ijbiomac.2017.07.150" @default.
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