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• W2741831087 startingPage "10783" @default.
• W2741831087 abstract "In Chlamydomonas reinhardtii, regulation of chloroplast gene expression is mainly post-transcriptional. It requires nucleus-encoded trans-acting protein factors for maturation/stabilization (M factors) or translation (T factors) of specific target mRNAs. We used long- and small-RNA sequencing to generate a detailed map of the transcriptome. Clusters of sRNAs marked the 5′ end of all mature mRNAs. Their absence in M-factor mutants reflects the protection of transcript 5′ end by the cognate factor. Enzymatic removal of 5′-triphosphates allowed identifying those cosRNA that mark a transcription start site. We detected another class of sRNAs derived from low abundance transcripts, antisense to mRNAs. The formation of antisense sRNAs required the presence of the complementary mRNA and was stimulated when translation was inhibited by chloramphenicol or lincomycin. We propose that they derive from degradation of double-stranded RNAs generated by pairing of antisense and sense transcripts, a process normally hindered by the traveling of the ribosomes. In addition, chloramphenicol treatment, by freezing ribosomes on the mRNA, caused the accumulation of 32–34 nt ribosome-protected fragments. Using this ‘in vivo ribosome footprinting’, we identified the function and molecular target of two candidate trans-acting factors." @default.
• W2741831087 created "2017-08-08" @default.
• W2741831087 creator A5003919815 @default.
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• W2741831087 creator A5083655714 @default.
• W2741831087 date "2017-08-02" @default.
• W2741831087 modified "2023-10-17" @default.
• W2741831087 title "Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism" @default.
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