FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2741831427> ?p ?o ?g. }

• W2741831427 endingPage "3744" @default.
• W2741831427 startingPage "3734" @default.
• W2741831427 abstract "Summary The degradation of the industrially produced and environmentally relevant phthalate esters by microorganisms is initiated by the hydrolysis to alcohols and phthalate (1,2‐dicarboxybenzene). In the absence of oxygen the further degradation of phthalate proceeds via activation to phthaloyl‐CoA followed by decarboxylation to benzoyl‐CoA. Here, we report on the first purification and characterization of a phthaloyl‐CoA decarboxylase (PCD) from the denitrifying Thauera chlorobenzoica . Hexameric PCD belongs to the UbiD‐family of (de)carboxylases and contains prenylated FMN (prFMN), K + and, unlike other UbiD‐like enzymes, Fe 2+ as cofactors. The latter is suggested to be involved in oxygen‐independent electron‐transfer during oxidative prFMN maturation. Either oxidation to the Fe 3+ ‐state in air or removal of K + by desalting resulted in >92% loss of both, prFMN and decarboxylation activity suggesting the presence of an active site prFMN/Fe 2+ /K + ‐complex in PCD. The PCD‐catalysed reaction was essentially irreversible: neither carboxylation of benzoyl‐CoA in the presence of 2 M bicarbonate, nor an isotope exchange of phthaloyl‐CoA with 13 C‐bicarbonate was observed. PCD differs in many aspects from prFMN‐containing UbiD‐like decarboxylases and serves as a biochemically accessible model for the large number of UbiD‐like (de)carboxylases that play key roles in the anaerobic degradation of environmentally relevant aromatic pollutants." @default.
• W2741831427 created "2017-08-08" @default.
• W2741831427 creator A5006923741 @default.
• W2741831427 creator A5008218698 @default.
• W2741831427 creator A5023813456 @default.
• W2741831427 creator A5033190394 @default.
• W2741831427 creator A5044195433 @default.
• W2741831427 creator A5049778197 @default.
• W2741831427 creator A5077445480 @default.
• W2741831427 date "2017-08-14" @default.
• W2741831427 modified "2023-10-18" @default.
• W2741831427 title "Phthaloyl‐coenzyme A decarboxylase from <i>Thauera chlorobenzoica</i>: the prenylated flavin‐, K⁺‐ and Fe²⁺‐dependent key enzyme of anaerobic phthalate degradation" @default.
• W2741831427 cites W1497636640 @default.
• W2741831427 cites W1506317292 @default.
• W2741831427 cites W1513007390 @default.
• W2741831427 cites W1518940859 @default.
• W2741831427 cites W1526671183 @default.
• W2741831427 cites W1579366804 @default.
• W2741831427 cites W1620207664 @default.
• W2741831427 cites W1928441380 @default.
• W2741831427 cites W1941706098 @default.
• W2741831427 cites W1958029566 @default.
• W2741831427 cites W1969364897 @default.
• W2741831427 cites W1970706674 @default.
• W2741831427 cites W1973668045 @default.
• W2741831427 cites W2008323358 @default.
• W2741831427 cites W2015094321 @default.
• W2741831427 cites W2019005194 @default.
• W2741831427 cites W2020551149 @default.
• W2741831427 cites W2048687101 @default.
• W2741831427 cites W2058594842 @default.
• W2741831427 cites W2067359298 @default.
• W2741831427 cites W2070744836 @default.
• W2741831427 cites W2074580449 @default.
• W2741831427 cites W2078325715 @default.
• W2741831427 cites W2082511599 @default.
• W2741831427 cites W2089344799 @default.
• W2741831427 cites W2096938048 @default.
• W2741831427 cites W2114902713 @default.
• W2741831427 cites W2117982960 @default.
• W2741831427 cites W2120828617 @default.
• W2741831427 cites W2140096309 @default.
• W2741831427 cites W2155277410 @default.
• W2741831427 cites W2156602461 @default.
• W2741831427 cites W2256166090 @default.
• W2741831427 cites W2293468919 @default.
• W2741831427 cites W2295080910 @default.
• W2741831427 cites W2410252166 @default.
• W2741831427 cites W2460308434 @default.
• W2741831427 cites W2460670816 @default.
• W2741831427 cites W2518508533 @default.
• W2741831427 cites W2568201064 @default.
• W2741831427 cites W2909775845 @default.
• W2741831427 doi "https://doi.org/10.1111/1462-2920.13875" @default.
• W2741831427 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28752942" @default.
• W2741831427 hasPublicationYear "2017" @default.
• W2741831427 type Work @default.
• W2741831427 sameAs 2741831427 @default.
• W2741831427 citedByCount "26" @default.
• W2741831427 countsByYear W27418314272017 @default.
• W2741831427 countsByYear W27418314272018 @default.
• W2741831427 countsByYear W27418314272019 @default.
• W2741831427 countsByYear W27418314272020 @default.
• W2741831427 countsByYear W27418314272021 @default.
• W2741831427 countsByYear W27418314272022 @default.
• W2741831427 countsByYear W27418314272023 @default.
• W2741831427 crossrefType "journal-article" @default.
• W2741831427 hasAuthorship W2741831427A5006923741 @default.
• W2741831427 hasAuthorship W2741831427A5008218698 @default.
• W2741831427 hasAuthorship W2741831427A5023813456 @default.
• W2741831427 hasAuthorship W2741831427A5033190394 @default.
• W2741831427 hasAuthorship W2741831427A5044195433 @default.
• W2741831427 hasAuthorship W2741831427A5049778197 @default.
• W2741831427 hasAuthorship W2741831427A5077445480 @default.
• W2741831427 hasConcept C161790260 @default.
• W2741831427 hasConcept C178790620 @default.
• W2741831427 hasConcept C181199279 @default.
• W2741831427 hasConcept C185592680 @default.
• W2741831427 hasConcept C197957613 @default.
• W2741831427 hasConcept C2779634684 @default.
• W2741831427 hasConcept C2780595602 @default.
• W2741831427 hasConcept C2781112554 @default.
• W2741831427 hasConcept C33093398 @default.
• W2741831427 hasConcept C55493867 @default.
• W2741831427 hasConcept C86803240 @default.
• W2741831427 hasConceptScore W2741831427C161790260 @default.
• W2741831427 hasConceptScore W2741831427C178790620 @default.
• W2741831427 hasConceptScore W2741831427C181199279 @default.
• W2741831427 hasConceptScore W2741831427C185592680 @default.
• W2741831427 hasConceptScore W2741831427C197957613 @default.
• W2741831427 hasConceptScore W2741831427C2779634684 @default.
• W2741831427 hasConceptScore W2741831427C2780595602 @default.
• W2741831427 hasConceptScore W2741831427C2781112554 @default.
• W2741831427 hasConceptScore W2741831427C33093398 @default.
• W2741831427 hasConceptScore W2741831427C55493867 @default.
• W2741831427 hasConceptScore W2741831427C86803240 @default.
• W2741831427 hasFunder F4320320879 @default.
• W2741831427 hasIssue "9" @default.

• next