FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2742148490> ?p ?o ?g. }

• W2742148490 abstract "Abstract The human leukocyte antigen (HLA) system is a gene family that encodes the human major histocompatibility complex (MHC). HLA-B is the most polymorphic gene in the MHC class I region, comprised of 4,765 HLA-B alleles (IPD-IMGT/HLA Database Release 3.28). Many HLA-B alleles have been associated with adverse drug reactions and disease risks, and we are interested in developing efficient methods for analysis of HLA alleles in this context. Here we describe an approach to HLA-B typing using multiplexed next generation sequencing on the MinION™ nanopore sequencer (Oxford Nanopore Technologies), combined with data analysis with the SeqNext-HLA software package (JSI Medical Systems GmbH, Ettenheim, Germany). The nanopore sequencer offers the advantages of long-read capability and single molecule reads, which can facilitate effective haplotyping. We developed this method using reference samples of known HLA-B type as well as individuals of New Zealand Māori or Pacific Island (Polynesian) descent, because HLA-B diversity in these populations is not well understood. We demonstrate here that nanopore sequencing of barcoded, pooled, 943 bp polymerase chain reaction (PCR) amplicons of 49 DNA samples, on one R9.4 flowcell (Oxford Nanopore Technologies), generated ample read depth for all samples. Sequence analysis using SeqNext-HLA software assigned HLA-B alleles to all samples at high-resolution with very little ambiguity. Our PCR-based next generation sequencing method is a scaleable and efficient approach for genotyping HLA-B and potentially any other HLA locus. Finally, we report our findings on HLA-B genotypes of this cohort, which adds to our understanding of HLA-B allele frequencies among Māori and Polynesian people." @default.
• W2742148490 created "2017-08-08" @default.
• W2742148490 creator A5001451653 @default.
• W2742148490 creator A5008504111 @default.
• W2742148490 creator A5010818082 @default.
• W2742148490 creator A5048129626 @default.
• W2742148490 creator A5061552694 @default.
• W2742148490 creator A5086843719 @default.
• W2742148490 date "2017-07-27" @default.
• W2742148490 modified "2023-09-24" @default.
• W2742148490 title "Multiplexed nanopore sequencing of HLA-B locus in Māori and Polynesian samples" @default.
• W2742148490 cites W1899908265 @default.
• W2742148490 cites W1964305251 @default.
• W2742148490 cites W1968789418 @default.
• W2742148490 cites W1971924684 @default.
• W2742148490 cites W1984706655 @default.
• W2742148490 cites W1986362755 @default.
• W2742148490 cites W1995318624 @default.
• W2742148490 cites W2019630785 @default.
• W2742148490 cites W2026861756 @default.
• W2742148490 cites W2044645437 @default.
• W2742148490 cites W2048787676 @default.
• W2742148490 cites W2059858676 @default.
• W2742148490 cites W2071728953 @default.
• W2742148490 cites W2091364441 @default.
• W2742148490 cites W2096475535 @default.
• W2742148490 cites W2105172077 @default.
• W2742148490 cites W2124499964 @default.
• W2742148490 cites W2141040548 @default.
• W2742148490 cites W2159600532 @default.
• W2742148490 cites W2159835983 @default.
• W2742148490 cites W2168990146 @default.
• W2742148490 cites W2205365896 @default.
• W2742148490 cites W2579779953 @default.
• W2742148490 cites W2588041338 @default.
• W2742148490 cites W4236035733 @default.
• W2742148490 cites W4240870042 @default.
• W2742148490 cites W4246627738 @default.
• W2742148490 cites W625410429 @default.
• W2742148490 doi "https://doi.org/10.1101/169078" @default.
• W2742148490 hasPublicationYear "2017" @default.
• W2742148490 type Work @default.
• W2742148490 sameAs 2742148490 @default.
• W2742148490 citedByCount "2" @default.
• W2742148490 countsByYear W27421484902018 @default.
• W2742148490 crossrefType "posted-content" @default.
• W2742148490 hasAuthorship W2742148490A5001451653 @default.
• W2742148490 hasAuthorship W2742148490A5008504111 @default.
• W2742148490 hasAuthorship W2742148490A5010818082 @default.
• W2742148490 hasAuthorship W2742148490A5048129626 @default.
• W2742148490 hasAuthorship W2742148490A5061552694 @default.
• W2742148490 hasAuthorship W2742148490A5086843719 @default.
• W2742148490 hasBestOaLocation W27421484901 @default.
• W2742148490 hasConcept C104317684 @default.
• W2742148490 hasConcept C126513998 @default.
• W2742148490 hasConcept C135763542 @default.
• W2742148490 hasConcept C141795571 @default.
• W2742148490 hasConcept C147483822 @default.
• W2742148490 hasConcept C151730666 @default.
• W2742148490 hasConcept C171250308 @default.
• W2742148490 hasConcept C180754005 @default.
• W2742148490 hasConcept C188280979 @default.
• W2742148490 hasConcept C192562407 @default.
• W2742148490 hasConcept C207936829 @default.
• W2742148490 hasConcept C2779343474 @default.
• W2742148490 hasConcept C2779927696 @default.
• W2742148490 hasConcept C31467283 @default.
• W2742148490 hasConcept C41007239 @default.
• W2742148490 hasConcept C49105822 @default.
• W2742148490 hasConcept C51679486 @default.
• W2742148490 hasConcept C54355233 @default.
• W2742148490 hasConcept C70712619 @default.
• W2742148490 hasConcept C70721500 @default.
• W2742148490 hasConcept C8185291 @default.
• W2742148490 hasConcept C84597430 @default.
• W2742148490 hasConcept C86803240 @default.
• W2742148490 hasConceptScore W2742148490C104317684 @default.
• W2742148490 hasConceptScore W2742148490C126513998 @default.
• W2742148490 hasConceptScore W2742148490C135763542 @default.
• W2742148490 hasConceptScore W2742148490C141795571 @default.
• W2742148490 hasConceptScore W2742148490C147483822 @default.
• W2742148490 hasConceptScore W2742148490C151730666 @default.
• W2742148490 hasConceptScore W2742148490C171250308 @default.
• W2742148490 hasConceptScore W2742148490C180754005 @default.
• W2742148490 hasConceptScore W2742148490C188280979 @default.
• W2742148490 hasConceptScore W2742148490C192562407 @default.
• W2742148490 hasConceptScore W2742148490C207936829 @default.
• W2742148490 hasConceptScore W2742148490C2779343474 @default.
• W2742148490 hasConceptScore W2742148490C2779927696 @default.
• W2742148490 hasConceptScore W2742148490C31467283 @default.
• W2742148490 hasConceptScore W2742148490C41007239 @default.
• W2742148490 hasConceptScore W2742148490C49105822 @default.
• W2742148490 hasConceptScore W2742148490C51679486 @default.
• W2742148490 hasConceptScore W2742148490C54355233 @default.
• W2742148490 hasConceptScore W2742148490C70712619 @default.
• W2742148490 hasConceptScore W2742148490C70721500 @default.
• W2742148490 hasConceptScore W2742148490C8185291 @default.
• W2742148490 hasConceptScore W2742148490C84597430 @default.
• W2742148490 hasConceptScore W2742148490C86803240 @default.
• W2742148490 hasLocation W27421484901 @default.

• next