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- W2742310719 abstract "This chapter discusses the preparation of RNA and ribosomes from yeast. Most methods used for the preparation of RNA and ribosomes from extracts of mammalian cells or bacteria can be directly applied either to yeast spheroplasts or to disrupted yeast cells. Yeast RNA can be radioactively labeled in vivo with purine and pyrimidine bases, phosphate-32P, and methyl-labeled methionine. Yeasts are able to utilize purines and pyrimidines added to the medium, although the free bases are not normal intermediates in the biosynthesis of nucleotides. Uracil and adenine are the bases most commonly used to label RNA. It is difficult to evaluate the usefulness for preparative purposes of the various labeling protocols, because the specific activities of the labeled RNAs are rarely determined. In the chapter, preparation of ribosomal subunits and polyribosomes and preparation of the high-molecular-weight rRNAs and low-molecular-weight rRNAs are discussed. An overview of polyacrylamide gel electrophoresis of low-molecular-weight RNAs and high-molecular-weight rRNA precursors is also presented in the chapter." @default.
- W2742310719 created "2017-08-17" @default.
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- W2742310719 date "1975-01-01" @default.
- W2742310719 modified "2023-09-25" @default.
- W2742310719 title "Chapter 4 Preparation of RNA and Ribosomes from Yeast" @default.
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- W2742310719 doi "https://doi.org/10.1016/s0091-679x(08)60951-6" @default.
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