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- W2742435190 abstract "Objective: To explore effect of fluorochloridone on primary co-cultured sertoli-germ cell of rat and its possible mechanism. Methods: primary co-cultured sertoli-germ cell was made by two steps of en-zyme digestion with SD rat testes, after 24h of Sertoli-germ cell isolation, A 0.1% DMSO solvent control group and three FLC exposure groups (10-6、10-7、10-8 mol/L) were selected, cultured cell for 24h, then MTT assay and index detection of oxidative stress were performed. Results: The mortality of primary co-cultured sertoli-germ cell exposed to 10-6 mol/L FLC was significantly higher than control group and FLC exposure groups (10-7、10-8 mol/L) (P<0.05) . 10-6 mol/L FLC reduced enzyme activity of CAT, SOD and GSH. Px, depressed GSH level, el-evated MDA level, and had significant difference than control group and FLC exposure groups(10-7、10-8 mol/L) (P<0.05) . 10-7 mol/L FLC decreased enzyme activity of CAT than control group, depressed enzyme activity of SOD than 10-8 mol/L FLC, lowered GSH level than control group and FLC exposure groups (10-8 mol/L) , and had statistical difference (P<0.05) . Conclusion: FLC can damage primary co-cultured sertoli-germ cell of rat, its possible mechanism is relevant to oxidative stress." @default.
- W2742435190 created "2017-08-17" @default.
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- W2742435190 date "2016-11-20" @default.
- W2742435190 modified "2023-09-23" @default.
- W2742435190 title "[Effect of fluorochloridone on oxidative stress in primary co-cultured sertoli-germ cell of rat]." @default.
- W2742435190 doi "https://doi.org/10.3760/cma.j.issn.1001-9391.2016.11.001" @default.
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