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• W2742700903 abstract "We present protein-PAINT - the implementation of the general principles of PAINT (Point Accumulation for Imaging in Nanoscale Topography) for live-cell protein labeling. Our method employs the specific binding of cell-permeable fluorogenic dyes to genetically encoded protein tags. We engineered three mutants of the bacterial lipocalin Blc that possess different affinities to a fluorogenic dye and exhibit a strong increase in fluorescence intensity upon binding. This allows for rapid labeling and washout of intracellular targets on a time scale from seconds to a few minutes. We demonstrate an order of magnitude higher photostability of the fluorescence signal in comparison with spectrally similar fluorescent proteins. Protein-PAINT ensures prolonged super-resolution fluorescence microscopy of living cells in both single molecule detection and stimulated emission depletion regimes." @default.
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• W2742700903 date "2017-01-01" @default.
• W2742700903 modified "2023-10-16" @default.
• W2742700903 title "Protein labeling for live cell fluorescence microscopy with a highly photostable renewable signal" @default.
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• W2742700903 doi "https://doi.org/10.1039/c7sc01628j" @default.
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