FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2742757861> ?p ?o ?g. }

• W2742757861 endingPage "91257" @default.
• W2742757861 startingPage "91248" @default.
• W2742757861 abstract "// Mengzhe Guo 1, * , Xiao Li 1, * , Liyan Zhang 1, * , Dantong Liu 1 , Wencheng Du 1 , Dengyang Yin 3 , Nan Lyu 4 , Guangyu Zhao 3 , Cheng Guo 2 and Daoquan Tang 1 1 Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu 221004, China 2 Cancer Institute, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China 3 Jingjiang People’s Hospital, Jingjiang, Jiangsu 214500, China 4 Xuzhou Central Hospital, Xuzhou, Jiangsu 221004, China * These authors contributed equally to this work and are co-first authors Correspondence to: Daoquan Tang, email: tdq993@hotmail.com Cheng Guo, email: cheng_guo@zju.edu.cn Keywords: liquid chromatography mass spectrometry, DNA demethylation, breast cancer, derivatization, 5-methylcytosine Received: June 22, 2017      Accepted: July 26, 2017      Published: August 09, 2017 ABSTRACT The DNA demethylation pathway has been discovered to play a significant role in DNA epigenetics. This pathway removes the methyl group from cytosine, which is involved in the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine (5-hmC) by ten-eleven translocation (TET) proteins. Then, 5-hmC can be iteratively oxidized to generate 5-formylcytosine (5-foC) and 5-carboxylcytosine (5-caC). However, 5-hmC, 5-foC, and 5-caC are hardly detected due to their low content. In this study, we have developed a LC-HRMS method coupled with derivatization to accurately and simultaneously quantify 5-mC levels, along with its oxidation products in genomic DNA. Derivatization was carried out using 4-dimethylamino benzoic anhydride, which has been shown to improve separation and enhance the detection sensitivity. Finally, we successfully applied this method towards the quantification of 5-mC, 5-hmC, 5-foC, and 5-caC in genomic DNA isolated from both human breast cancer tissue and tumor-adjacent normal tissue. We show that 5-foC and 5-caC are increased in tumor tissue. In addition, the levels of 5-mC, 5-hmC, 5-foC, and 5-caC measured in tumor tissue versus tumor-adjacent tissue were found to be distinct among different classifications. This suggests that cytosine modifiers could be used as potential biomarkers for determining the stage of development of breast cancer, as well as prognosis." @default.
• W2742757861 created "2017-08-17" @default.
• W2742757861 creator A5009173399 @default.
• W2742757861 creator A5033585312 @default.
• W2742757861 creator A5035339773 @default.
• W2742757861 creator A5045086561 @default.
• W2742757861 creator A5049689249 @default.
• W2742757861 creator A5050769390 @default.
• W2742757861 creator A5066218991 @default.
• W2742757861 creator A5066900811 @default.
• W2742757861 creator A5070379325 @default.
• W2742757861 creator A5076454806 @default.
• W2742757861 date "2017-08-09" @default.
• W2742757861 modified "2023-10-12" @default.
• W2742757861 title "Accurate quantification of 5-Methylcytosine, 5-Hydroxymethylcytosine, 5-Formylcytosine, and 5-Carboxylcytosine in genomic DNA from breast cancer by chemical derivatization coupled with ultra performance liquid chromatography- electrospray quadrupole time of flight mass spectrometry analysis" @default.
• W2742757861 cites W1963339255 @default.
• W2742757861 cites W1967064280 @default.
• W2742757861 cites W1968584162 @default.
• W2742757861 cites W1981543052 @default.
• W2742757861 cites W1984066495 @default.
• W2742757861 cites W1987753375 @default.
• W2742757861 cites W1989457196 @default.
• W2742757861 cites W1993145571 @default.
• W2742757861 cites W1997453048 @default.
• W2742757861 cites W2004078197 @default.
• W2742757861 cites W2004172747 @default.
• W2742757861 cites W2011052948 @default.
• W2742757861 cites W2024162802 @default.
• W2742757861 cites W2029004484 @default.
• W2742757861 cites W2031712869 @default.
• W2742757861 cites W2032814455 @default.
• W2742757861 cites W2038449573 @default.
• W2742757861 cites W2038722690 @default.
• W2742757861 cites W2039053594 @default.
• W2742757861 cites W2043472013 @default.
• W2742757861 cites W2069932077 @default.
• W2742757861 cites W2102083376 @default.
• W2742757861 cites W2106878505 @default.
• W2742757861 cites W2108664872 @default.
• W2742757861 cites W2113665333 @default.
• W2742757861 cites W2124049725 @default.
• W2742757861 cites W2140681432 @default.
• W2742757861 cites W2152497434 @default.
• W2742757861 cites W2156386262 @default.
• W2742757861 cites W2171490882 @default.
• W2742757861 cites W2329238656 @default.
• W2742757861 cites W2509538052 @default.
• W2742757861 cites W2529263829 @default.
• W2742757861 cites W2551357583 @default.
• W2742757861 cites W2553084674 @default.
• W2742757861 cites W2611995393 @default.
• W2742757861 cites W4235160748 @default.
• W2742757861 doi "https://doi.org/10.18632/oncotarget.20093" @default.
• W2742757861 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5710920" @default.
• W2742757861 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29207640" @default.
• W2742757861 hasPublicationYear "2017" @default.
• W2742757861 type Work @default.
• W2742757861 sameAs 2742757861 @default.
• W2742757861 citedByCount "21" @default.
• W2742757861 countsByYear W27427578612018 @default.
• W2742757861 countsByYear W27427578612019 @default.
• W2742757861 countsByYear W27427578612020 @default.
• W2742757861 countsByYear W27427578612021 @default.
• W2742757861 countsByYear W27427578612022 @default.
• W2742757861 countsByYear W27427578612023 @default.
• W2742757861 crossrefType "journal-article" @default.
• W2742757861 hasAuthorship W2742757861A5009173399 @default.
• W2742757861 hasAuthorship W2742757861A5033585312 @default.
• W2742757861 hasAuthorship W2742757861A5035339773 @default.
• W2742757861 hasAuthorship W2742757861A5045086561 @default.
• W2742757861 hasAuthorship W2742757861A5049689249 @default.
• W2742757861 hasAuthorship W2742757861A5050769390 @default.
• W2742757861 hasAuthorship W2742757861A5066218991 @default.
• W2742757861 hasAuthorship W2742757861A5066900811 @default.
• W2742757861 hasAuthorship W2742757861A5070379325 @default.
• W2742757861 hasAuthorship W2742757861A5076454806 @default.
• W2742757861 hasBestOaLocation W27427578611 @default.
• W2742757861 hasConcept C104317684 @default.
• W2742757861 hasConcept C150194340 @default.
• W2742757861 hasConcept C179998833 @default.
• W2742757861 hasConcept C185592680 @default.
• W2742757861 hasConcept C190727270 @default.
• W2742757861 hasConcept C2776923573 @default.
• W2742757861 hasConcept C2778901553 @default.
• W2742757861 hasConcept C43617362 @default.
• W2742757861 hasConcept C55493867 @default.
• W2742757861 hasConcept C71924100 @default.
• W2742757861 hasConceptScore W2742757861C104317684 @default.
• W2742757861 hasConceptScore W2742757861C150194340 @default.
• W2742757861 hasConceptScore W2742757861C179998833 @default.
• W2742757861 hasConceptScore W2742757861C185592680 @default.
• W2742757861 hasConceptScore W2742757861C190727270 @default.
• W2742757861 hasConceptScore W2742757861C2776923573 @default.
• W2742757861 hasConceptScore W2742757861C2778901553 @default.
• W2742757861 hasConceptScore W2742757861C43617362 @default.
• W2742757861 hasConceptScore W2742757861C55493867 @default.
• W2742757861 hasConceptScore W2742757861C71924100 @default.
• W2742757861 hasIssue "53" @default.

• next