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- W2742768711 abstract "In this thesis we study the horizontal gene transfer (HGT) of gene coding mikimopine synthase (mis gene) from Agrobacterium rhizogenes to recipients - species within plant genera Linaria and Nicotiana. mis gene is a part of agrobacterial T-DNA plasmid. The role of mis gene is to synthesize agrobactrium-specific nutrients inside of host cells. Using in silico analyses of mis gene sequences, gained from databases or via detection and amplification in wet lab, we show three possible fates of the mis gene evolution after HGT event. In the case of genus Linaria, mis gene started to degenerate shortly after the incorporation into the plant genome. The fate of mis gene among Nicotiana species is different. mis gene of N. glauca has untouched open reading frame, is transcriptionally active, and is evolving under strong selective pressure. mis gene probably possesses some benefitial effects for N. glauca, is beeing domesticated, and/or maybe is beeing neofunctionalized. mis gene within the genome of N. tabacum and N. tomentosiformis has a shift in open reading frame followed by the presence of nonsense mutations. However, all analyses again predict evolution under selective pressures and a possible role in plant immunity defence mechanisms against pathogenes. To verify host attempts to silence transposable elements, that agrobacterial T-DNA actually is, via cytosine methylation, we created an user friendly application for the design of primers dedicated for bisulfite sequencing. The tool is named BisPrimer and has optimized default settings which can be customized by user. BisPrimer designs primers for bisulfite treated plant DNA, as well as for bisulfite treated mammalian DNA, alongside an entire uploaded sequence or only for an user defined part." @default.
- W2742768711 created "2017-08-17" @default.
- W2742768711 creator A5013295095 @default.
- W2742768711 date "2017-01-01" @default.
- W2742768711 modified "2023-09-23" @default.
- W2742768711 title "Evoluční aspekty horizontálního genového přenosu u rostlin" @default.
- W2742768711 hasPublicationYear "2017" @default.
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