Matches in SemOpenAlex for { <https://semopenalex.org/work/W2743025654> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2743025654 endingPage "475" @default.
- W2743025654 startingPage "471" @default.
- W2743025654 abstract "To construct eukaryotic expression vector of human P2X7gene and transfect HEK293 cells so as to establish stable HEK293 cell line.P2X7 gene was amplified by polymerase chain reaction from the human brain P2X7 cDNA and inserted into a vector pEGFP-N1 to construct a recombinant plasmidcalled pEGFP-N1/P2X7. The correct recombinant plasmid was transfected into HEK293 cells by X-fect transfection reagent. The cell line stably expressing EGFP tagged-P2X7 gene were established by screening with G418 and fluorescence microscope. The expression levels and localization of human P2X7 in HEK293 cells was identified by flow cytometry, Western blot and laser scanning confocal microscope.The recombinant plasmid pEGFP-N1/P2X7 was constructed correctly and the stable HEK293 cell line expressing EGFP tagged-P2X7 fusion protein was established. Both Western blot and flow cytometry revealed the higher expression of humanP2X7 in the stably transfected HEK293 cells. Under the laser scanning confocal microscope the EGFP tagged-P2X7 fusion protein was located on the membrane of HEK293 cells.The eukaryotic expressing vector of pEGFP-N1/P2X7 is successfully constructed and the HEK293 cell line stably expressing P2X7-EGFP fusion protein is established which have provided solid experimental foundation for further studies on the structure and function of P2X7 ionic channel." @default.
- W2743025654 created "2017-08-17" @default.
- W2743025654 creator A5003816728 @default.
- W2743025654 creator A5018853571 @default.
- W2743025654 creator A5032539937 @default.
- W2743025654 creator A5051829452 @default.
- W2743025654 creator A5059877994 @default.
- W2743025654 creator A5079998068 @default.
- W2743025654 creator A5081025433 @default.
- W2743025654 date "2016-05-08" @default.
- W2743025654 modified "2023-09-28" @default.
- W2743025654 title "[Construction of eukaryotic expressing vector of human P2X7 and establishment of stable transfectant cell line]." @default.
- W2743025654 doi "https://doi.org/10.13459/j.cnki.cjap.2016.05.022" @default.
- W2743025654 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29931855" @default.
- W2743025654 hasPublicationYear "2016" @default.
- W2743025654 type Work @default.
- W2743025654 sameAs 2743025654 @default.
- W2743025654 citedByCount "0" @default.
- W2743025654 crossrefType "journal-article" @default.
- W2743025654 hasAuthorship W2743025654A5003816728 @default.
- W2743025654 hasAuthorship W2743025654A5018853571 @default.
- W2743025654 hasAuthorship W2743025654A5032539937 @default.
- W2743025654 hasAuthorship W2743025654A5051829452 @default.
- W2743025654 hasAuthorship W2743025654A5059877994 @default.
- W2743025654 hasAuthorship W2743025654A5079998068 @default.
- W2743025654 hasAuthorship W2743025654A5081025433 @default.
- W2743025654 hasConcept C104317684 @default.
- W2743025654 hasConcept C123894998 @default.
- W2743025654 hasConcept C142613039 @default.
- W2743025654 hasConcept C153911025 @default.
- W2743025654 hasConcept C174510640 @default.
- W2743025654 hasConcept C187882448 @default.
- W2743025654 hasConcept C22744801 @default.
- W2743025654 hasConcept C2776415932 @default.
- W2743025654 hasConcept C40767141 @default.
- W2743025654 hasConcept C54009773 @default.
- W2743025654 hasConcept C54355233 @default.
- W2743025654 hasConcept C553184892 @default.
- W2743025654 hasConcept C81885089 @default.
- W2743025654 hasConcept C86803240 @default.
- W2743025654 hasConceptScore W2743025654C104317684 @default.
- W2743025654 hasConceptScore W2743025654C123894998 @default.
- W2743025654 hasConceptScore W2743025654C142613039 @default.
- W2743025654 hasConceptScore W2743025654C153911025 @default.
- W2743025654 hasConceptScore W2743025654C174510640 @default.
- W2743025654 hasConceptScore W2743025654C187882448 @default.
- W2743025654 hasConceptScore W2743025654C22744801 @default.
- W2743025654 hasConceptScore W2743025654C2776415932 @default.
- W2743025654 hasConceptScore W2743025654C40767141 @default.
- W2743025654 hasConceptScore W2743025654C54009773 @default.
- W2743025654 hasConceptScore W2743025654C54355233 @default.
- W2743025654 hasConceptScore W2743025654C553184892 @default.
- W2743025654 hasConceptScore W2743025654C81885089 @default.
- W2743025654 hasConceptScore W2743025654C86803240 @default.
- W2743025654 hasIssue "5" @default.
- W2743025654 hasLocation W27430256541 @default.
- W2743025654 hasOpenAccess W2743025654 @default.
- W2743025654 hasPrimaryLocation W27430256541 @default.
- W2743025654 hasRelatedWork W2359175313 @default.
- W2743025654 hasRelatedWork W2361843014 @default.
- W2743025654 hasRelatedWork W2363386379 @default.
- W2743025654 hasRelatedWork W2380100330 @default.
- W2743025654 hasRelatedWork W2383434177 @default.
- W2743025654 hasRelatedWork W2387731041 @default.
- W2743025654 hasRelatedWork W2401891876 @default.
- W2743025654 hasRelatedWork W2408811821 @default.
- W2743025654 hasRelatedWork W2989893422 @default.
- W2743025654 hasRelatedWork W172312785 @default.
- W2743025654 hasVolume "32" @default.
- W2743025654 isParatext "false" @default.
- W2743025654 isRetracted "false" @default.
- W2743025654 magId "2743025654" @default.
- W2743025654 workType "article" @default.