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- W2743162024 abstract "This chapter examines the three-dimensional reconstruction of single molecules. Biological macromolecules have defined structures that are uniquely related to their metabolic functions. Two approaches have been proposed for three-dimensional reconstruction of single molecules. In the first approach a series of views of an individual molecule is picked from micrographs of tilting series. The molecule is reconstructed from these views by Fourier techniques. It is found that as no averaging is applied in this method, the reconstructed mass-density distribution represents the molecule together with the surrounding stain and the attached support film structure. Individual Fourier coefficients of single-particle projections, because of their high noise content, cannot be analyzed and compared individually. The proper way to compare features and assess the reproducibility of projection measurements or their Fourier transforms is to use global measures such as correlation functions. It is suggested that the resolution of the reconstruction should be matched to the resolution limit created by all the artifacts." @default.
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- W2743162024 date "1981-01-01" @default.
- W2743162024 modified "2023-09-25" @default.
- W2743162024 title "chapter 16 Three-Dimensional Reconstruction of Single Molecules" @default.
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- W2743162024 doi "https://doi.org/10.1016/s0091-679x(08)61882-8" @default.
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