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- W2743260012 abstract "The ethanolic extract of the barks of Xylocarpus moluccensis (Lamk.) Roem (Family: Meliaceae) was evaluated for its antioxidant activities and quantification of bioactive polyphenols by HPLC. ABTS radical scavenging activity, reducing power, total antioxidant capacity, nitric oxide scavenging activity, total phenolic content, and total flavonoid content were used for determining antioxidant activities of the bark extract. In ABTS radical scavenging assay, the extract showed 99.03% inhibition at the concentration of 5 μg/ml. The IC50 of ethanol extract (3.05 μg/ml) in ABTS scavenging was significant compared to the standard ascorbic acid (13.25 μg/ml) The maximum absorbance for reducing power assay was found to 1.3801 at 250 μg/ml while compared to 1.1115 and 1.0310 for standard ascorbic acid and BHA respectively. Total antioxidant capacity was measured, based on the reduction of Mo(VI) to Mo(V) by the extract and found to be 685 mg of ascorbic acid/gm of extract. The ethanol extract showed maximum scavenging activity of 41.33% at 250 μg/ml, where as ascorbic acid at the same concentration exhibited 27.79% inhibition. The total phenolic amount was significant and calculated as 778.95 mg/g of gallic acid equivalent. The total flavonoid content was also calculated in ethanolic crude extract (4.92 mg/g of quercetin equivalent). In HPLC assay, catechin hydrate was quantified in significant amount (500.45 mg/100 g of dry extract) and moderate concentration of epicatechin 79.20 mg/100 g of extract were also determined for the first time.Therefore, the obtained results tend to suggest the antioxidant activities with high concentration of catechin hydrate in the extract of the barks of Xylocarpus moluccensis and justify its use in folkloric remedies. Keywords: ABTS radical scavenging activity; Total antioxidant capacity; Catechin hydrate; Epicatechin." @default.
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- W2743260012 date "2017-03-19" @default.
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- W2743260012 title "Antioxidant Activities and HPLC Assay of Bioactive Polyphenols of the Ethanolic Bark Extract of Xylocarpus moluccensis (Lamk.) Roem" @default.
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