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• W2743262892 abstract "This chapter focuses on the fractionation of chromatin by buoyant density–gradient sedimentation in metrizamide. Most of the methods that have been used in attempts to fractionate chromatin have been designed to take advantage of the differences observed in vivo between heterochromatin and euchromatin—namely, the former is granulated and highly condensed, while the latter appears to exist mainly as uncondensed, extended fibrils. It has been realized that certain X-ray contrast media and their derivatives possess many of the attributes required of a density–gradient solute suitable for the isopycnic sedimentation of unfixed nucleoproteins. Metrizamide is totally nonionic in aqueous solutions and has been most widely exploited for experiments involving the isopycnic banding of macromolecules, macromolecular complexes, cell organelles, whole cells, and viruses. The chapter discusses the use of metrizamide for the isopycnic sedimentation of unfixed chromatin and describes the important properties of metrizamide. Formation of gradients of metrizamide and fractionation and analysis of metrizamide gradients are described in the chapter." @default.
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• W2743262892 date "1978-01-01" @default.
• W2743262892 modified "2023-10-01" @default.
• W2743262892 title "Chapter 2 Fractionation of Chromatin by Buoyant Density-Gradient Sedimentation in Metrizamide" @default.
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• W2743262892 doi "https://doi.org/10.1016/s0091-679x(08)60130-2" @default.
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