FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2743284581> ?p ?o ?g. }

• W2743284581 endingPage "10533" @default.
• W2743284581 startingPage "10518" @default.
• W2743284581 abstract "The yeast 2-μm plasmid is a remarkable genetic parasite, managing efficient maintenance at high-copy number with minimal impact on the host. Equal partitioning of the plasmid upon host cell division requires plasmid proteins Rep1 and Rep2 and the plasmid STB locus. The Rep proteins and the plasmid-encoded Raf protein also regulate plasmid gene transcription. In this study, protein interaction assays, sequence analyses and mutational approaches were used to identify domains and residues in Rep2 and Raf required for association with Rep1 and Rep2 and to delineate the Rep2 DNA-binding domain. Rep2 and Raf displayed similarities in interactions with Rep1 and Rep2, in having Rep1 promote their STB association in vivo, and in stabilizing Rep protein levels. Rep2 mutants impaired for self-association were competent for transcriptional repression while those deficient for Rep1 association were not. Surprisingly, Rep2 mutants impaired for either Rep1 interaction or self-association were able to maintain efficient plasmid inheritance provided Raf was present and competent for Rep protein interaction. Our findings provide insight into the Rep protein complexes required for partitioning and transcriptional repression, and suggest that in addition to its transcriptional function, Raf stabilization of Rep partitioning proteins contributes to the remarkable persistence of the 2-μm plasmid." @default.
• W2743284581 created "2017-08-17" @default.
• W2743284581 creator A5010302753 @default.
• W2743284581 creator A5030639374 @default.
• W2743284581 creator A5036718794 @default.
• W2743284581 creator A5049859810 @default.
• W2743284581 creator A5060258090 @default.
• W2743284581 creator A5090431341 @default.
• W2743284581 date "2017-08-09" @default.
• W2743284581 modified "2023-10-18" @default.
• W2743284581 title "The yeast 2-μm plasmid Raf protein contributes to plasmid inheritance by stabilizing the Rep1 and Rep2 partitioning proteins" @default.
• W2743284581 cites W1548068969 @default.
• W2743284581 cites W1834233095 @default.
• W2743284581 cites W185535816 @default.
• W2743284581 cites W1855382658 @default.
• W2743284581 cites W1898887825 @default.
• W2743284581 cites W1899785354 @default.
• W2743284581 cites W1969993146 @default.
• W2743284581 cites W1971798880 @default.
• W2743284581 cites W1982690685 @default.
• W2743284581 cites W1982741134 @default.
• W2743284581 cites W1984846896 @default.
• W2743284581 cites W1989779163 @default.
• W2743284581 cites W1990921346 @default.
• W2743284581 cites W2002285090 @default.
• W2743284581 cites W2007904006 @default.
• W2743284581 cites W2010653401 @default.
• W2743284581 cites W2010974877 @default.
• W2743284581 cites W2013292795 @default.
• W2743284581 cites W2014370060 @default.
• W2743284581 cites W2016531468 @default.
• W2743284581 cites W2023922223 @default.
• W2743284581 cites W2029476353 @default.
• W2743284581 cites W2046705158 @default.
• W2743284581 cites W2046712414 @default.
• W2743284581 cites W2050284387 @default.
• W2743284581 cites W2062796799 @default.
• W2743284581 cites W2064514648 @default.
• W2743284581 cites W2065494582 @default.
• W2743284581 cites W2068764665 @default.
• W2743284581 cites W2083191700 @default.
• W2743284581 cites W2097607684 @default.
• W2743284581 cites W2102254262 @default.
• W2743284581 cites W2109342168 @default.
• W2743284581 cites W2110251865 @default.
• W2743284581 cites W2113523085 @default.
• W2743284581 cites W2120571626 @default.
• W2743284581 cites W2126046383 @default.
• W2743284581 cites W2129099821 @default.
• W2743284581 cites W2137015675 @default.
• W2743284581 cites W2148910377 @default.
• W2743284581 cites W2169457212 @default.
• W2743284581 cites W2208864695 @default.
• W2743284581 cites W2245168818 @default.
• W2743284581 cites W2395995753 @default.
• W2743284581 cites W4236289065 @default.
• W2743284581 cites W4239406033 @default.
• W2743284581 doi "https://doi.org/10.1093/nar/gkx703" @default.
• W2743284581 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5737570" @default.
• W2743284581 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29048592" @default.
• W2743284581 hasPublicationYear "2017" @default.
• W2743284581 type Work @default.
• W2743284581 sameAs 2743284581 @default.
• W2743284581 citedByCount "11" @default.
• W2743284581 countsByYear W27432845812018 @default.
• W2743284581 countsByYear W27432845812019 @default.
• W2743284581 countsByYear W27432845812021 @default.
• W2743284581 countsByYear W27432845812022 @default.
• W2743284581 countsByYear W27432845812023 @default.
• W2743284581 crossrefType "journal-article" @default.
• W2743284581 hasAuthorship W2743284581A5010302753 @default.
• W2743284581 hasAuthorship W2743284581A5030639374 @default.
• W2743284581 hasAuthorship W2743284581A5036718794 @default.
• W2743284581 hasAuthorship W2743284581A5049859810 @default.
• W2743284581 hasAuthorship W2743284581A5060258090 @default.
• W2743284581 hasAuthorship W2743284581A5090431341 @default.
• W2743284581 hasBestOaLocation W27432845811 @default.
• W2743284581 hasConcept C104317684 @default.
• W2743284581 hasConcept C138885662 @default.
• W2743284581 hasConcept C143065580 @default.
• W2743284581 hasConcept C179926584 @default.
• W2743284581 hasConcept C22744801 @default.
• W2743284581 hasConcept C2777576037 @default.
• W2743284581 hasConcept C41895202 @default.
• W2743284581 hasConcept C54355233 @default.
• W2743284581 hasConcept C86803240 @default.
• W2743284581 hasConceptScore W2743284581C104317684 @default.
• W2743284581 hasConceptScore W2743284581C138885662 @default.
• W2743284581 hasConceptScore W2743284581C143065580 @default.
• W2743284581 hasConceptScore W2743284581C179926584 @default.
• W2743284581 hasConceptScore W2743284581C22744801 @default.
• W2743284581 hasConceptScore W2743284581C2777576037 @default.
• W2743284581 hasConceptScore W2743284581C41895202 @default.
• W2743284581 hasConceptScore W2743284581C54355233 @default.
• W2743284581 hasConceptScore W2743284581C86803240 @default.
• W2743284581 hasIssue "18" @default.
• W2743284581 hasLocation W27432845811 @default.
• W2743284581 hasLocation W27432845812 @default.

• next