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- W2743403133 abstract "The derivation of hepatocytes from human induced pluripotent stem cells (hiPSC) is of great interest for applications in pharmacological research. However, full maturation of hiPSC-derived hepatocytes has not yet been achieved in vitro. To improve hepatic differentiation, co-cultivation of hiPSC with human umbilical vein endothelial cells (HUVEC) during hepatic differentiation was investigated in this study. In the first step, different culture media variations based on hepatocyte culture medium (HCM) were tested in HUVEC mono-cultures to establish a suitable culture medium for co-culture experiments. Based on the results, two media variants were selected to differentiate hiPSC-derived definitive endodermal (DE) cells into mature hepatocytes with or without HUVEC addition. DE cells differentiated in mono-cultures in the presence of those media variants showed a significant increase (p < 0.05) in secretion of α-fetoprotein and in activities of cytochrome P450 (CYP) isoenzymes CYP2B6 and CYP3A4 as compared with cells differentiated in unmodified HCM used as control. Co-cultivation with HUVEC did not further improve the differentiation outcome. Thus, it can be concluded that the effect of the used medium outweighed the effect of HUVEC co-culture, emphasizing the importance of the culture medium composition for hiPSC differentiation." @default.
- W2743403133 created "2017-08-17" @default.
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- W2743403133 date "2017-08-07" @default.
- W2743403133 modified "2023-10-16" @default.
- W2743403133 title "Effects of Co-Culture Media on Hepatic Differentiation of hiPSC with or without HUVEC Co-Culture" @default.
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- W2743403133 cites W1978094951 @default.
- W2743403133 cites W1979114601 @default.
- W2743403133 cites W1980247655 @default.
- W2743403133 cites W1985342348 @default.
- W2743403133 cites W1985842788 @default.
- W2743403133 cites W1992837470 @default.
- W2743403133 cites W1994471498 @default.
- W2743403133 cites W1995765328 @default.
- W2743403133 cites W1995791162 @default.
- W2743403133 cites W1997086295 @default.
- W2743403133 cites W1997435071 @default.
- W2743403133 cites W1999914688 @default.
- W2743403133 cites W2000151960 @default.
- W2743403133 cites W2006838330 @default.
- W2743403133 cites W2010549748 @default.
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- W2743403133 cites W2046037975 @default.
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- W2743403133 cites W2055074097 @default.
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- W2743403133 cites W2149165594 @default.
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- W2743403133 doi "https://doi.org/10.3390/ijms18081724" @default.
- W2743403133 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5578114" @default.
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- W2743403133 hasPublicationYear "2017" @default.
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