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- W2743508932 endingPage "3126" @default.
- W2743508932 startingPage "3110" @default.
- W2743508932 abstract "The effect of the coupling approach (chemical by using carbodiimide chemistry, and enzymatic by using transglutaminase) of a hydrophilic ɛ-poly-L-lysine (ɛPL) and a structurally-hydrophobic oligo-acyl-lysyl (OAK) to a gelatine (GEL) macromolecule, and their antibacterial activity against Gram-negative E. coli and Gram-positive S. aureus bacteria, as well as cytotoxicity to human osteoblast cells was studied as potential macromolecules for biomedical applications. Different spectroscopic (ultraviolet-visible, infrared, fluorescence, and electron paramagnetic resonance) and separation (size-exclusion chromatography and capillary zone electrophoresis) techniques, as well as zeta-potential analysis were performed to confirm the ɛPL/OAK covalent coupling and to determine their amount and orientation of the immobilization. The highest and kinetically the fastest reduction of bacteria (≥77% against E. coli vs. ≥82% against S. aureus) was achieved with GEL functionalized with ɛPL/OAK by the chemical grafting-to approach being correlated with conformationally the highly-flexible ˝brush-like˝ orientation linkage of peptides, enable its targeted and rapid interactions with bacteria membrane. The up to 400-fold lower yield of OAKs being immobilized may be related also to its cationic charge and hydrophobic alkyl chain moieties, compared to more hydrophilic ɛPL easily causing random polymerization and self-conjugation. The ɛPL/OAK-functionalized GEL did not induce citotoxicity to osteoblasts, even at ∼25-fold higher concentration than bacterial minimum inhibitory (MIC) concentration of ɛPL/OAK. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3110–3126, 2017." @default.
- W2743508932 created "2017-08-17" @default.
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- W2743508932 date "2017-08-24" @default.
- W2743508932 modified "2023-09-27" @default.
- W2743508932 title "Antibacterial activity and cytotoxycity of gelatine-conjugated lysine-based peptides" @default.
- W2743508932 cites W128347032 @default.
- W2743508932 cites W1528991278 @default.
- W2743508932 cites W1532578585 @default.
- W2743508932 cites W1583078809 @default.
- W2743508932 cites W1658029431 @default.
- W2743508932 cites W1965496820 @default.
- W2743508932 cites W1970226578 @default.
- W2743508932 cites W1970377166 @default.
- W2743508932 cites W1971348435 @default.
- W2743508932 cites W1975452213 @default.
- W2743508932 cites W1979132970 @default.
- W2743508932 cites W1980751943 @default.
- W2743508932 cites W1983521156 @default.
- W2743508932 cites W1988857366 @default.
- W2743508932 cites W1989734247 @default.
- W2743508932 cites W1994694542 @default.
- W2743508932 cites W1994870882 @default.
- W2743508932 cites W1997152791 @default.
- W2743508932 cites W1997947008 @default.
- W2743508932 cites W2009119064 @default.
- W2743508932 cites W2018150201 @default.
- W2743508932 cites W2023424840 @default.
- W2743508932 cites W2024366939 @default.
- W2743508932 cites W2031573857 @default.
- W2743508932 cites W2035248785 @default.
- W2743508932 cites W2040976571 @default.
- W2743508932 cites W2046167890 @default.
- W2743508932 cites W2054602599 @default.
- W2743508932 cites W2056188017 @default.
- W2743508932 cites W2056975293 @default.
- W2743508932 cites W2059943881 @default.
- W2743508932 cites W2060943939 @default.
- W2743508932 cites W2064837586 @default.
- W2743508932 cites W2065967121 @default.
- W2743508932 cites W2067643078 @default.
- W2743508932 cites W2072084534 @default.
- W2743508932 cites W2075380940 @default.
- W2743508932 cites W2077718313 @default.
- W2743508932 cites W2093209335 @default.
- W2743508932 cites W2094455866 @default.
- W2743508932 cites W2094942535 @default.
- W2743508932 cites W2100705436 @default.
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- W2743508932 cites W2124690072 @default.
- W2743508932 cites W2135661305 @default.
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- W2743508932 cites W2137827489 @default.
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- W2743508932 cites W2155622354 @default.
- W2743508932 cites W2156413298 @default.
- W2743508932 cites W2159185781 @default.
- W2743508932 cites W2162524673 @default.
- W2743508932 cites W2163568950 @default.
- W2743508932 cites W2164898805 @default.
- W2743508932 cites W2170885324 @default.
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- W2743508932 cites W2339697811 @default.
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- W2743508932 cites W2506289253 @default.
- W2743508932 cites W4233471544 @default.
- W2743508932 cites W4242936818 @default.
- W2743508932 cites W4244732422 @default.
- W2743508932 cites W4248754741 @default.
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- W2743508932 doi "https://doi.org/10.1002/jbm.a.36164" @default.
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