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• W2743632014 abstract "Artificial guidance for cellular alignment is a hot topic in the field of tissue engineering. Most of the previous research has investigated single strain-induced cellular alignment on a cell-laden hydrogel by using complex experimental processes and mass controlling systems, which are usually associated with contamination issues. Thus, in this article, we propose a simple approach to building a gradient static strain using a fluidic chip with a plastic PDMS cover and a UV transparent glass substrate for the stimulation of cellular behavior in a 3D hydrogel. Overloading photo-patternable cell prepolymer in the fluidic chamber can generate a convex curved PDMS membrane on the cover. After UV crosslinking, through a concentric circular micropattern under the curved PDMS membrane, and buffer washing, a microenvironment for investigating cell behaviors under a variety of gradient strains is self-established in a single fluidic chip, without external instruments. NIH3T3 cells were demonstrated after observing the change in the cellular alignment trend under geometry guidance, in cooperation with strain stimulation, which varied from 15 - 65% on hydrogels. After a 3-day incubation, the hydrogel geometry dominated the cell alignment under low compressive strain, where cells aligned along the hydrogel elongation direction under high compressive strain. Between these, the cells showed random alignment due to the dissipation of the radical guidance of hydrogel elongation and the geometry guidance of the patterned hydrogel." @default.
• W2743632014 created "2017-08-17" @default.
• W2743632014 creator A5035193436 @default.
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• W2743632014 date "2017-08-08" @default.
• W2743632014 modified "2023-09-27" @default.
• W2743632014 title "Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel" @default.
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• W2743632014 doi "https://doi.org/10.3791/53715" @default.
• W2743632014 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5614120" @default.
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• W2743632014 hasPublicationYear "2017" @default.
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