Matches in SemOpenAlex for { <https://semopenalex.org/work/W2743882727> ?p ?o ?g. }
- W2743882727 abstract "Intralesional (IL) Mycobacterium bovis Bacillus Calmette-Guérin (BCG) has long been a relatively inexpensive therapy for inoperable cutaneous metastatic melanoma (CMM), although IL-BCG skin mechanisms remain understudied. We analyzed IL-BCG-treated CMM lesions combined with in vitro studies to further investigate BCG-altered pathways. Since macrophages play a pivotal role against both cancer and mycobacterial infections, we hypothesized BCG regulates macrophages to promote antitumor immunity. Tumor-associated macrophages (M2) infiltrate melanomas and impair antitumor immunity. BCG-treated, in vitro-polarized M2 (M2-BCG) showed transcriptional changes involving inflammation, immune cell recruitment, cross-talk and activation pathways. Mechanistic network analysis indicated M2-BCG potential to improve IFN-γ responses. Accordingly, frequency of IFN-γ-producing CD4+ T cells responding to M2-BCG vs. mock-treated M2 increased (p<0.05). Moreover, conditioned media from M2-BCG vs. M2 elevated the frequency of granzyme B-producing CD8+ tumor-infiltrating lymphocytes (TIL) facing autologous melanoma cell lines (p<0.01). Furthermore, transcriptome analysis of IL-BCG-injected CMM relative to uninjected lesions showed immune function prevalence, with the most enriched pathways representing T cell activation mechanisms. In vitro-infected MM-derived cell lines stimulated higher frequency of IFN-γ-producing TIL from the same melanoma (p<0.05). Our data suggests BCG favors antitumor responses in CMM through direct/indirect effects on tumor microenvironment cell types including macrophages, T cells and tumor itself." @default.
- W2743882727 created "2017-08-17" @default.
- W2743882727 creator A5009942708 @default.
- W2743882727 creator A5011604585 @default.
- W2743882727 creator A5016886577 @default.
- W2743882727 creator A5032417513 @default.
- W2743882727 creator A5035398490 @default.
- W2743882727 creator A5075444437 @default.
- W2743882727 creator A5080404941 @default.
- W2743882727 date "2017-08-11" @default.
- W2743882727 modified "2023-10-10" @default.
- W2743882727 title "Mycobacterium bovis Bacillus Calmette–Guérin Alters Melanoma Microenvironment Favoring Antitumor T Cell Responses and Improving M2 Macrophage Function" @default.
- W2743882727 cites W129007473 @default.
- W2743882727 cites W1530235393 @default.
- W2743882727 cites W1535567348 @default.
- W2743882727 cites W1543188088 @default.
- W2743882727 cites W1551344154 @default.
- W2743882727 cites W1576095056 @default.
- W2743882727 cites W1855030145 @default.
- W2743882727 cites W1963611242 @default.
- W2743882727 cites W1965130745 @default.
- W2743882727 cites W1968858038 @default.
- W2743882727 cites W1969353942 @default.
- W2743882727 cites W1984133971 @default.
- W2743882727 cites W1993641876 @default.
- W2743882727 cites W1996535081 @default.
- W2743882727 cites W1999498760 @default.
- W2743882727 cites W2001997364 @default.
- W2743882727 cites W2003998232 @default.
- W2743882727 cites W2004150448 @default.
- W2743882727 cites W2009323329 @default.
- W2743882727 cites W2016181667 @default.
- W2743882727 cites W2016612145 @default.
- W2743882727 cites W2020541351 @default.
- W2743882727 cites W2027848378 @default.
- W2743882727 cites W2029910544 @default.
- W2743882727 cites W2036481615 @default.
- W2743882727 cites W2037032239 @default.
- W2743882727 cites W2038781914 @default.
- W2743882727 cites W2046471818 @default.
- W2743882727 cites W2050343553 @default.
- W2743882727 cites W2055022031 @default.
- W2743882727 cites W2057577614 @default.
- W2743882727 cites W2058126719 @default.
- W2743882727 cites W2059403792 @default.
- W2743882727 cites W2061123122 @default.
- W2743882727 cites W2064648343 @default.
- W2743882727 cites W2065957571 @default.
- W2743882727 cites W2073747620 @default.
- W2743882727 cites W2074055091 @default.
- W2743882727 cites W2078011863 @default.
- W2743882727 cites W2087459726 @default.
- W2743882727 cites W2102436790 @default.
- W2743882727 cites W2102464966 @default.
- W2743882727 cites W2103017472 @default.
- W2743882727 cites W2103230271 @default.
- W2743882727 cites W2103753861 @default.
- W2743882727 cites W2104839914 @default.
- W2743882727 cites W2107009994 @default.
- W2743882727 cites W2108512542 @default.
- W2743882727 cites W2109274792 @default.
- W2743882727 cites W2109590683 @default.
- W2743882727 cites W2114496790 @default.
- W2743882727 cites W2116041602 @default.
- W2743882727 cites W2117281169 @default.
- W2743882727 cites W2120426523 @default.
- W2743882727 cites W2123469465 @default.
- W2743882727 cites W2123721949 @default.
- W2743882727 cites W2125369953 @default.
- W2743882727 cites W2127559758 @default.
- W2743882727 cites W2134791218 @default.
- W2743882727 cites W2135915203 @default.
- W2743882727 cites W2140380746 @default.
- W2743882727 cites W2143210160 @default.
- W2743882727 cites W2143437930 @default.
- W2743882727 cites W2150076846 @default.
- W2743882727 cites W2150770343 @default.
- W2743882727 cites W2152705444 @default.
- W2743882727 cites W2157513403 @default.
- W2743882727 cites W2163175900 @default.
- W2743882727 cites W2171446213 @default.
- W2743882727 cites W2171490333 @default.
- W2743882727 cites W2171878279 @default.
- W2743882727 cites W2183585286 @default.
- W2743882727 cites W2239561092 @default.
- W2743882727 cites W2266594975 @default.
- W2743882727 cites W2287156124 @default.
- W2743882727 cites W229763590 @default.
- W2743882727 cites W2318644551 @default.
- W2743882727 cites W2330366745 @default.
- W2743882727 cites W2397170433 @default.
- W2743882727 cites W2398235143 @default.
- W2743882727 cites W2401718791 @default.
- W2743882727 cites W2412517238 @default.
- W2743882727 cites W2423606312 @default.
- W2743882727 cites W2592502679 @default.
- W2743882727 cites W2604183104 @default.
- W2743882727 cites W4243799633 @default.
- W2743882727 cites W4248728884 @default.
- W2743882727 cites W82937767 @default.