FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2744023218> ?p ?o ?g. }

• W2744023218 endingPage "e0182752" @default.
• W2744023218 startingPage "e0182752" @default.
• W2744023218 abstract "Dysregulated human eccrine sweat glands can negatively impact the quality-of-life of people suffering from disorders like hyperhidrosis. Inability of sweating can even result in serious health effects in humans affected by anhidrosis. The underlying mechanisms must be elucidated and a reliable in vitro test system for drug screening must be developed. Here we describe a novel organotypic three-dimensional (3D) sweat gland model made of primary human eccrine sweat gland cells. Initial experiments revealed that eccrine sweat gland cells in a two-dimensional (2D) culture lose typical physiological markers. To resemble the in vivo situation as close as possible, we applied the hanging drop cultivation technology regaining most of the markers when cultured in its natural spherical environment. To compare the organotypic 3D sweat gland model versus human sweat glands in vivo, we compared markers relevant for the eccrine sweat gland using transcriptomic and proteomic analysis. Comparing the marker profile, a high in vitro-in vivo correlation was shown. Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5), muscarinic acetylcholine receptor M3 (CHRM3), Na+-K+-Cl- cotransporter 1 (NKCC1), calcium-activated chloride channel anoctamin-1 (ANO1/TMEM16A), and aquaporin-5 (AQP5) are found at significant expression levels in the 3D model. Moreover, cholinergic stimulation with acetylcholine or pilocarpine leads to calcium influx monitored in a calcium flux assay. Cholinergic stimulation cannot be achieved with the sweat gland cell line NCL-SG3 used as a sweat gland model system. Our results show clear benefits of the organotypic 3D sweat gland model versus 2D cultures in terms of the expression of essential eccrine sweat gland key regulators and in the physiological response to stimulation. Taken together, this novel organotypic 3D sweat gland model shows a good in vitro-in vivo correlation and is an appropriate alternative for screening of potential bioactives regulating the sweat mechanism." @default.
• W2744023218 created "2017-08-17" @default.
• W2744023218 creator A5019512557 @default.
• W2744023218 creator A5024518402 @default.
• W2744023218 creator A5029355773 @default.
• W2744023218 creator A5033137487 @default.
• W2744023218 creator A5034993611 @default.
• W2744023218 creator A5035675071 @default.
• W2744023218 creator A5040178005 @default.
• W2744023218 creator A5041599934 @default.
• W2744023218 date "2017-08-10" @default.
• W2744023218 modified "2023-10-13" @default.
• W2744023218 title "A novel organotypic 3D sweat gland model with physiological functionality" @default.
• W2744023218 cites W1603017005 @default.
• W2744023218 cites W1646243120 @default.
• W2744023218 cites W183116773 @default.
• W2744023218 cites W1924256936 @default.
• W2744023218 cites W1964135106 @default.
• W2744023218 cites W1971363623 @default.
• W2744023218 cites W1971934199 @default.
• W2744023218 cites W1986806238 @default.
• W2744023218 cites W2002667291 @default.
• W2744023218 cites W2004905658 @default.
• W2744023218 cites W2007177947 @default.
• W2744023218 cites W2007261241 @default.
• W2744023218 cites W2015448455 @default.
• W2744023218 cites W2016337018 @default.
• W2744023218 cites W2018479772 @default.
• W2744023218 cites W2018566932 @default.
• W2744023218 cites W2025458210 @default.
• W2744023218 cites W2026840218 @default.
• W2744023218 cites W2027501393 @default.
• W2744023218 cites W2027733541 @default.
• W2744023218 cites W2028918602 @default.
• W2744023218 cites W2029920529 @default.
• W2744023218 cites W2033375715 @default.
• W2744023218 cites W2037805890 @default.
• W2744023218 cites W2037972438 @default.
• W2744023218 cites W2041434086 @default.
• W2744023218 cites W2043504958 @default.
• W2744023218 cites W2049241172 @default.
• W2744023218 cites W2051197697 @default.
• W2744023218 cites W2053197056 @default.
• W2744023218 cites W2066178574 @default.
• W2744023218 cites W2068551838 @default.
• W2744023218 cites W2070307389 @default.
• W2744023218 cites W2070976096 @default.
• W2744023218 cites W2071014556 @default.
• W2744023218 cites W2072329257 @default.
• W2744023218 cites W2073865475 @default.
• W2744023218 cites W2074004858 @default.
• W2744023218 cites W2075001151 @default.
• W2744023218 cites W2076531413 @default.
• W2744023218 cites W2078792089 @default.
• W2744023218 cites W2079827027 @default.
• W2744023218 cites W2086316164 @default.
• W2744023218 cites W2097695518 @default.
• W2744023218 cites W2117669260 @default.
• W2744023218 cites W2155175184 @default.
• W2744023218 cites W2161286017 @default.
• W2744023218 cites W2171326656 @default.
• W2744023218 cites W2172183744 @default.
• W2744023218 cites W2173724542 @default.
• W2744023218 cites W2175811952 @default.
• W2744023218 cites W2184321826 @default.
• W2744023218 cites W2236643502 @default.
• W2744023218 cites W2255750302 @default.
• W2744023218 cites W2276824282 @default.
• W2744023218 cites W2292269715 @default.
• W2744023218 cites W2062478164 @default.
• W2744023218 doi "https://doi.org/10.1371/journal.pone.0182752" @default.
• W2744023218 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5552089" @default.
• W2744023218 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28796813" @default.
• W2744023218 hasPublicationYear "2017" @default.
• W2744023218 type Work @default.
• W2744023218 sameAs 2744023218 @default.
• W2744023218 citedByCount "26" @default.
• W2744023218 countsByYear W27440232182018 @default.
• W2744023218 countsByYear W27440232182019 @default.
• W2744023218 countsByYear W27440232182020 @default.
• W2744023218 countsByYear W27440232182021 @default.
• W2744023218 countsByYear W27440232182022 @default.
• W2744023218 countsByYear W27440232182023 @default.
• W2744023218 crossrefType "journal-article" @default.
• W2744023218 hasAuthorship W2744023218A5019512557 @default.
• W2744023218 hasAuthorship W2744023218A5024518402 @default.
• W2744023218 hasAuthorship W2744023218A5029355773 @default.
• W2744023218 hasAuthorship W2744023218A5033137487 @default.
• W2744023218 hasAuthorship W2744023218A5034993611 @default.
• W2744023218 hasAuthorship W2744023218A5035675071 @default.
• W2744023218 hasAuthorship W2744023218A5040178005 @default.
• W2744023218 hasAuthorship W2744023218A5041599934 @default.
• W2744023218 hasBestOaLocation W27440232181 @default.
• W2744023218 hasConcept C126322002 @default.
• W2744023218 hasConcept C134018914 @default.
• W2744023218 hasConcept C150903083 @default.
• W2744023218 hasConcept C169760540 @default.
• W2744023218 hasConcept C207001950 @default.

• next